Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
Western blot analysis of Survivin in extracts from HeLa cells 48 hours following mock transfection, transfection with nonspecific (control) siRNA or transfection with Survivin siRNA, using Survivin monoclonal antibody (Product # MA5-15207) and a p42 MAP Kinase (Erk2) polyclonal antibody. The Survivin antibody confirms silencing of Survivin expression, and the p42 MAPK antibody is used to control for protein loading and siRNA specificity.
|Tested species reactivity||Human, Primate|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic peptide corresponding to the amino-terminal sequence of human survivin|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Contains||<0.02% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
It is not recommended to aliquot this antibody.
This antibody is not cross-reactive with other types of apoptotic inhibitor proteins.
This gene is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors yet low in adult tissues. Antisense transcripts are involved in the regulation of this gene's expression. At least four transcript variants encoding distinct isoforms have been found for this gene, but the full-length natures of only three of them have been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
apoptosis inhibitor 4; apoptosis inhibitor survivin; baculoviral IAP repeat-containing protein 5; survivin variant 3 alpha
API4; BIRC5; EPR-1; IAP4