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|Tested species reactivity||Dog, Cat, Guinea pig, Hamster, Human, Mouse, Rat|
|Published species reactivity||Rat, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant human survivin, full-length.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||-20°C or -80°C if preferred|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||1:10-1:500|
|Flow Cytometry (Flow)||Assay-Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay-Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:500|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 2 publications below|
For IHC, prior antigen retrieval (pressure cooking) is recommended for cytoplasmic and nuclear detection of Survivin.
Suggested positive control: Hela whole cell extract, antigen standard for BIRC5 (transient overexpression lysate).
Regulated inhibition of programmed cell death (apoptosis) preserves normal homeostasis and tissue and organ morphogenesis. Aberrations in this process contribute to human diseases and cancer by abnormally prolonging cell viability. Recently, several apoptosis inhibitors related to the baculovirus iap gene have been found in various species, including human. IAP proteins contain one/three Cys/His baculovirus IAP repeats plus a c-terminus RING finger and are thought to block an evolutionary conserved step in apoptosis. Survivin encodes a structurally unique inhibitor of apoptosis (IAP). Survivin expression is turned off during fetal development and is not found in non-neoplastic adult human tissues. Survivin becomes abundantly re-expressed in transformed cells and in all of the most common cancers of lung, colon, pancreas, breast and prostate in vivo. Survivin appears to be situated at the crossroads of cell death and cell division, governing a checkpoint involved in cytokinesis while also suppressing apoptosis. Survivin is also abundantly expressed in brain tissues (astrocytes and some neurons) of adult rats following traumatic brain injury. Survivin has been found co-expressed with NeuN (mature neuronal marker) and PCNA (a cell cycle protein). Survivin might affect regulation of neural cell proliferative responses after brain injury.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Hepatitis B virus X protein accelerates hepatocarcinogenesis with partner survivin through modulating miR-520b and HBXIP.
PA1-16836 was used in western blot to investigate hepatocarcinogenesis mediated by hepatitis B virus X protein
|Zhang W,Lu Z,Kong G,Gao Y,Wang T,Wang Q,Cai N,Wang H,Liu F,Ye L,Zhang X||Molecular cancer (13:null)||2014|
NR4A1 enhances neural survival following oxygen and glucose deprivation: an in vitro study.
PA1-16836 was used in western blot to study the ability of NR4A1 to protect against neural damage induced by oxygen and glucose deprivation
|Xiao G,Sun T,Songming C,Cao Y||Journal of the neurological sciences (330:78)||2013|
API4; Apoptosis Inhibitor 4; Apoptosis inhibitor survivin; Baculoviral IAP repeat containing 5; baculoviral IAP repeat-containing 5; baculoviral IAP repeat-containing 5 (survivin); Baculoviral IAP repeat-containing protein 5; BIRC 5; EPR 1; IAP4; survivin; survivin variant 3 alpha; SVV; TIAP
AAC-11; AP14; API4; BIRC5; EPR-1; IAP4; survivin40; TIAP