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Survivin Antibody (740055MP647) in ICC/IF
Immunofluorescence analysis of Survivin was performed using Survivin Recombinant Mouse Monoclonal Antibody (STLALYV), Alexa Fluor™ Plus 647 (Product # 740055MP647) on HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 mins, permeabilized with 0.5% Triton X-100 for 10 mins and blocked with 2% BSA for 1 hr at room temperature. The cells were stained with Survivin Recombinant Mouse Monoclonal Antibody (STLALYV), Alexa Fluor™ Plus 647 (Product # 740055MP647, 1:200) in 0.1% BSA, incubated at 4 degree Celsius overnight. Survivin Re... View More
Product Details
740055MP647
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b, kappa
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
STLALYV
Conjugate
Excitation/Emission Max
658/675 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
50 µg
Purification
Protein A/G
Storage buffer
proprietary buffer, pH 6.3-7.3
Contains
0.008% Bromonitrodioxane, 0.008% Methylisothiazolone
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
RRID
Product Specific Information
Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 658 nm; Emission: 675 nm; Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Survivin (IAP4) is the smallest member of the Inhibitors of Apoptosis Protein (IAP) gene family. The IAPs are involved in multiple cell functions such as cell signaling, cell division, metabolism, and exhibits differential expression in nearly all human cancers, but not in most normal tissues. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but the Survivin gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors yet low in adult tissues. Antisense transcripts are involved in the regulation of survivin's gene expression. Survivin is expressed in the G2/M phase of the cell cycle in a cycle-regulated manner. At the beginning of mitosis, survivin associates with microtubules of the mitotic spindle in a specific and saturable reaction that is regulated by microtubule dynamics. Disruption of survivin-microtubule interactions results in loss of survivin's anti-apoptosis function and increased caspase-3 activity, a mechanism involved in cell death, during mitosis. Survivin may counteract a default induction of apoptosis in G2/M phase. Survivin is also abundantly expressed in brain tissues (astrocytes and some neurons) of adult rats following traumatic brain injury. Survivin has been found co-expressed with NeuN (mature neuronal marker) and PCNA (a cell cycle protein). Survivin might affect regulation of neural cell proliferative responses after brain injury. At least four transcript variants encoding distinct isoforms have been found for this gene, but the full-length natures of only three of them have been determined. The overexpression of survivin in cancer may overcome this apoptotic checkpoint and favor aberrant progression of transformed cells through mitosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: anti-apoptosis protein; IAP; Apoptosis inhibitor 4; Apoptosis inhibitor survivin; baculoviral IAP repeat-containing 5; Baculoviral IAP repeat-containing protein 5; BIRC 5; BIRC5; EPR 1; IAP; SVV; unnamed protein product
Gene Aliases: API4; BIRC5; EPR-1; IAP4
UniProt ID: (Human) O15392
Entrez Gene ID: (Human) 332
cysteine-type endopeptidase inhibitor activity
protein binding
microtubule binding
zinc ion binding
tubulin binding
enzyme binding
peptidase inhibitor activity
small GTPase binding
identical protein binding
protein homodimerization activity
cysteine-type endopeptidase inhibitor activity involved in apoptotic process
metal ion binding
protein heterodimerization activity
cobalt ion binding
chaperone binding
G2/M transition of mitotic cell cycle
mitotic cell cycle
mitotic cytokinesis
apoptotic process
mitotic spindle organization
chromosome segregation
mitotic spindle assembly checkpoint
sensory perception of sound
positive regulation of cell proliferation
protein complex localization
positive regulation of exit from mitosis
negative regulation of apoptotic process
negative regulation of transcription, DNA-templated
positive regulation of mitotic cell cycle
mitotic spindle midzone assembly
cell division
establishment of chromosome localization
positive regulation of mitotic cell cycle spindle assembly checkpoint
mitotic spindle assembly
positive regulation of mitotic sister chromatid separation
positive regulation of attachment of mitotic spindle microtubules to kinetochore
positive regulation of mitotic cytokinesis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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