|Tested species reactivity||Yeast|
|Published species reactivity||Yeast|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant Swi6 from E. coli.|
|Storage buffer||whole serum|
|Contains||0.09% sodium azide|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1:2000 - 1:10000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|ChIP assay (ChIP)||See 2 publications below|
PA1-4977 detects Swi6 from S. pombe samples.
PA1-4977 has been successfully used in Western blot and immunoprecimmunoprecipitationitation applications. Western blot analysis of crude extract of S. pombe detects a protein with an apparent molecular size of 53 kD.
Swi6 protein of fission yeast is a functional and structural homolog of HP1 (Heterochromatin Protein 1) of animals and is involved in the formation of heterochromatin structure by binding to centromere, telomere and silent mating-type locus. It is also involved in silencing the genes and sister chromatid cohesion by binding to histone H3 methylated at Lys9 and the cohesin subunit Psc3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The Mi-2 homolog Mit1 actively positions nucleosomes within heterochromatin to suppress transcription.
PA1-4977 was used in ChIP assay to study the mechanism by which Mit1 remodels chromatin to suppress gene transcription
|Creamer KM,Job G,Shanker S,Neale GA,Lin YC,Bartholomew B,Partridge JF||Molecular and cellular biology (34:2046)||2014|
Distinct roles for Sir2 and RNAi in centromeric heterochromatin nucleation, spreading and maintenance.
PA1-4977 was used in ChIP assay to study the roles of RNAi and the Sir2 histone deacetylase in the establishment and integrity of centromeric heterochromatin
|Buscaino A,Lejeune E,Audergon P,Hamilton G,Pidoux A,Allshire RC||The EMBO journal (32:1250)||2013|