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|Tested species reactivity||Bovine, Human, Mouse, Rat|
|Host / Isotype||Mouse / IgM|
|Immunogen||Synaptic vesicle-containing fractions, immunoprecipitated from human brain homogenates.|
|Purification||Ammonium sulfate precipitation|
|Storage buffer||PBS, pH 7.2, with 0.1mM PMSF, 50% glycerol|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||Assay Dependent|
|Western Blot (WB)||1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-26000 detects Synaptophysin from human, mouse, rat, and bovine samples.
MA1-26000 has been successfully used in immunoprecipitation, Immunohistochemistry, and Western blot procedures. MA1-26000 detects a band of ~38 kDa. Postive control of mouse and rat brain tissue extract suggested. No reactivity was detected in mouse liver extracts.
The MA1-26000 immunogen is synaptic vesicle-containing fractions, immunoprecipitated from human brain homogenates.
Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles.
Synaptophysin is a 38kDa glycoprotein present in the membrane of neuronal presynaptic vesicles in brain, spinal cord, retina, vesicles of adrenal medulla, neuromuscular junctions, and endocrine cells. It is also expressed by neuroendocrine cells throughout the body, both normal and neoplastic. Synaptophysin is a useful marker for the identification of normal neuroendocrine cells and neuroendocrine neoplasms. This antibody reacts with neuroendocrine cells of human adrenal medulla, carotid body, skin, pituitary gland, thyroid, lung, pancreas, and gastointestinal mucosa. It also reacts with a wide spectrum of neuroendocrine neoplasms of neural type including neuroblastomas, ganglioneuroblastomas, ganglioneuromas, pheochromocytomas, chromaffin, and non-chromaffin paragangliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
MRXSYP; synaptobrevin 1; synaptobrevin-1; VAMP-1; vesicle-associated membrane protein 1; vesicle-associated membrane protein 1 (synaptobrevin 1)
BOS_6013; lew; SPAX1; Syb-1; SYB1; VAMP-1; VAMP1