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Immunofluorescence analysis of Synaptophysin was done on Primary Rat Hippocampus neuronal cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with ABfinity™ Synaptophysin Recombinant Rabbit Oligoclonal Antibody (710532) at a dilution of 1:400 in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Flour® 488 Goat Anti-Rabbit IgG Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). F-actin (Panel b: red) was stained with Alexa Fluor® 594 Phalloidin (A12381). Nuclei (Panel c: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). Panel d is a merged image showing dendritic localization. The images were captured using a Nikon microscope at 20X magnification.
|Tested species reactivity||Human, Rat|
|Host / Isotype||Rabbit / IgG|
|Contains||0.09% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Synaptophysin (p38) is an integral membrane protein of small synaptic vesicles in brain and endocrine cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
major synaptic vesicle protein P38; MRXSYP; synaptophysin
MRX96; MRXSYP; SYP; Syp1