Immunohistochemistry analysis of Synaptophysin showing staining in the cytoplasm of paraffin-embedded human pancreas tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Synaptophysin Mouse Monoclonal Antibody (MA5-11575) diluted in 3% BSA-PBS at a dilution of 1:100 for 1 hour at 37ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||A synthetic peptide encoding a region near the C-terminal end of the synaptophysin protein|
|Storage buffer||tissue culture supernatant|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-11575 targets Synaptophysin in IHC (P) applications and shows reactivity with Human and Rat samples.
The MA5-11575 immunogen is a synthetic peptide encoding a region near the C-terminal end of the synaptophysin protein.
Synaptophysin (p38) is an integral membrane protein of small synaptic vesicles in brain and endocrine cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Papillary glioneuronal tumor--a rare entity: report of four cases and brief review of literature.
MA5-11575 was used in immunohistochemistry to report on four cases of papillary glioneuronal tumor
|Agarwal S,Sharma MC,Singh G,Suri V,Sarkar C,Garg A,Kumar R,Chandra PS||Child's nervous system : ChNS : official journal of the International Society for Pediatric Neurosurgery (28:1897)||2012|
Invasive lobular carcinoma with extracellular mucin as a distinct variant of lobular carcinoma: a case report.
MA5-11575 was used in immunohistochemistry to report on a case of invasive lobular carcinoma with extracellular mucin
|Haltas H,Bayrak R,Yenidunya S,Kosehan D,Sen M,Akin K||Diagnostic pathology (7:null)||2012|
Desmoplastic small round cell tumor of the submandibular gland--a rare but distinctive primary salivary gland neoplasm.
MA5-11575 was used in immunohistochemistry to report on a case of desmoplastic small round cell tumor of the submandibular gland
|Yin WH,Guo SP,Yang HY,Chan JK||Human pathology (41:438)||2010|
Carcinoid-like pattern in melanoma: report of 4 cases.
MA5-11575 was used in immunohistochemistry to report on four cases of carcinoid-like pattern in melanoma
|Kacerovska D,Michal M,Sosna B,Cempirkova D,Ambrus M,Richtr P,Danis D,Zelger B,Kazakov DV||The American Journal of dermatopathology (31:542)||2009|
Cerebellar liponeurocytoma/lipidized medulloblastoma.
MA5-11575 was used in immunohistochemistry to report on a case of cerebellar liponeurocytoma/lipidized medulloblastoma
|Aker FV,Ozkara S,Eren P,Peker O,Arma¿an S,Hakan T||Journal of neuro-oncology (71:53)||2005|
Generation of structures formed by lens and retinal cells differentiating from embryonic stem cells.
MA5-11575 was used in immunocytochemistry to develop a culture system in which lens, neural retina and pigmented retina structures can be induced from embryonic stem cells
|Hirano M,Yamamoto A,Yoshimura N,Tokunaga T,Motohashi T,Ishizaki K,Yoshida H,Okazaki K,Yamazaki H,Hayashi S,Kunisada T||Developmental dynamics : an official publication of the American Association of Anatomists (228:664)||2003|