Invitrogen

Synaptophysin Monoclonal Antibody (7H12)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

View all (85) Synaptophysin antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

Synaptophysin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded human small intestine, myenteric plexus tissues using SYP monoclonal antibody (Product # MA5-15623).

Synaptophysin Antibody in Western Blot (WB) — Western blot was performed using Anti-Synaptophysin Monoclonal Antibody (7H12) (Product # MA5-15623) and a 38kDa band corresponding to Synaptophysin was observed across in IMR-32, PC-12, Neuro-2a, Mouse Brain and Rat Brain, but not in SK-BR-3, BJ, NIH-3T3, Mouse Kidney and Rat Kidney. Whole cell extracts (30 µg lysate) of IMR-32 (Lane 1), SK-BR-3 (Lane 2), BJ (Lane 3), PC-12 (Lane 4), Neuro-2a (Lane 5), NIH-3T3 (Lane 6), Mouse Brain (Lane 7), Mouse Kidney (Lane 8), Rat Brain (Lane 9), Rat Kidney (Lane 10) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Synaptophysin Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across the tested cell lines and tissues owing to their inherent genetic constitution. Relative expression of Synaptophysin was observed in IMR-32, PC-12, Neuro-2a, Mouse Brain and Rat Brain, but not in SK-BR-3, BJ, NIH-3T3, Mouse Kidney and Rat Kidney using Anti-Synaptophysin Monoclonal Antibody (7H12) (Product # MA5-15623) in Western Blot. {RE}

Product Details

MA5-15623

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (IHC)	1:200-1:1,000	-
ELISA (ELISA)	1:10,000	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	7H12
Immunogen	Purified recombinant fragment of SYP expressed in E. Coli.
Conjugate	Unconjugated
Form	Liquid
Concentration	Conc. Not Determined
Storage buffer	ascites
Contains	0.03% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_10986932

Product Specific Information

MA5-15623 targets SYP in IHC and WB applications and shows reactivity with Human and Rat samples.

The MA5-15623 immunogen is purified recombinant fragment of SYP expressed in E. Coli.

MA5-15623 detects SYP which has a predicted molecular weight of approximately 33.8kDa.

Target Information

Synaptophysin is a calcium-binding and integral membrane glycoprotein present in presynaptic vesicles in almost all neurons. Synaptophysin has four transmembrane domains and it forms a complex with dynamin at high calcium concentrations suggesting an involvement in synaptic vesicle endocytosis. It is also involved in the regulation of short-term and long-term synaptic plasticity. Synaptophysin is currently the most widely used marker for nerve terminals and for differentiating neuroendocrine tumors. Mutations in the gene can result in mental retardation, X-linked 96.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.