Invitrogen

Synaptophysin Monoclonal Antibody (SY38)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

3 Published Figures

5 References

View all (92) Synaptophysin antibodies

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Protocols

Questions & Answers

Tech Support

Synaptophysin Antibody in Western Blot (WB)

FIGURE: 1 / 8

Synaptophysin Antibody (MA1-213) in WB

Western blot was performed using Anti-Synaptophysin Monoclonal Antibody (SY38) (Product # MA1-213) and a 33 kDa band corresponding to Synaptophysin was observed across Mouse Brain and Rat Brain. Tissue extracts (30 µg lysate) of Mouse Heart (Lane 1), Mouse Liver (Lane 2), Mouse Brain (Lane 3) and Rat Brain (Lane 4) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0342BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). T... View More

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Synaptophysin Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA)) — Immunohistochemistry was performed on human cerebellum and human breast tissue. Tissue was deparaffinized with xylene, followed by rehydration in sequential washes of 100% ethanol, 95% ethanol, 80% ethanol, and water. To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0) and heated for 20 min. Following antigen retrieval, tissues were blocked in a 10% goat serum (Product # 31872) in wash buffer solution for 30 minutes at room temperature and endogenous peroxidase activity quenched with Peroxidase Suppressor (Product # 35000). Tissue was then probed with a Synaptophysin mouse monoclonal antibody (Product # MA1-213) at a dilution of 1:200 in 10% goat serum in wash buffer for 1 hour at room temperature in a humidified chamber. Negative control tissue received no primary antibody. Tissues were washed extensively with PBST, and detection was performed using a SuperBoost™ goat anti-mouse Poly HRP secondary antibody reagent (Product # B40961) followed by colorimetric detection using DAB Quanto (Product # TA-125-QHDX). Tissues were then counterstained with hematoxylin and prepped for mounting and imaging.

Synaptophysin Antibody in Flow Cytometry (Flow) — Flow cytometry analysis of Synaptophysin was done on PC12 cells. The cells were fixed, permeabilized and stained with a Synaptophysin mouse monoclonal antibody (Product # MA1-213, red histogram) or Mouse IgG isotype control (Product # 31903, blue histogram) at a dilution of 1:500. Black histogram represents negative control unstained cell population. After incubation of the primary antibody on ice for an hour, the cells were stained with a Goat anti-Mouse IgG (H+L) Secondary Antibody, Dylight 680 conjugate (Product # 35519) at a dilution of 1:50 for at least 30 minutes on ice. A representative 10,000 cells were acquired for each sample.

Synaptophysin Antibody in Immunohistochemistry (IHC) — Confocal microscopy of immunofluorescence staining for antibodies against human nucleoli (NM-95), human cytoplasm (STEM121), Synaptophysin (Syn; pre-synaptic terminal vesicle protein), and post-synaptic density (PSD-95) in a region with organoid implant taken from a mouse directly after the last electrophysiology recording at 11 weeks post-implantation. DAPI (blue) was used as counterstain for cell nuclei. a Co-localization of Syn (red) and PSD-95 (green) with human cells along the boundary (delineated in pink) of the organoid implant (Org) and visual cortex (Visual, arrowheads). b Human cells were observed surrounded by Syn, PSD-95, and mouse cells (arrowheads) in a region at the organoid implant and retrosplenial cortex (RSC) boundary (delineated in pink). c Human cells (arrowheads) were observed surrounded by PSD-95 and Syn, traveling along the corpus callosum (CC). The boundary between CC and visual cortex is delineated in pink. Note the smaller density of Syn and PSD-95, which is characteristic of the corpus callosum due to longer myelinated axonal projections and less synaptic terminals. d Puncta positive for Syn (green) and negative for hSyn (red) were defined as mouse pre-synaptic terminals. The number of mouse pre-synaptic puncta was high at the boundary of the organoid implant ( d , hollow arrowheads) but still existed at the center of the implant ( e , hollow arrowheads). Solid arrowheads in ( d , e ) indicate puncta positive for both hSyn and Syn, representing hum

Synaptophysin Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC) — FIGURE 7 Impaired rod maturation in PDE6B patient retinal organoids. (A) Representative immunostaining pictures of the presynaptic marker synaptophysin and rhodopsin (RHO) in retinal organoids. Bar: 25 mum. (B) Amplified presynaptic formation of rod photoreceptors indicated with arrows in (A) . Bar: 25 mum. (C) Colocalization of RHO and synaptophysin. (D) Representative immunostaining pictures of the cilium marker ARL13B and RHO in retinal organoids at D230. Bar: 25 mum. Boxes were amplified images. White arrows indicated definite signal of ARL13B represent cilia. Bar: 6.25 mum. (E) Statistic analysis of cilia number in retinal organoids at D230. Statistical results are the mean values +- SD of 3 organoids (3 images each) (D230) of two independent differentiations from the control and patient. Two-tailed t -test, *** P < 0.001.

Synaptophysin Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissues tested owing to their inherent genetic constitution. Relative expression of Synaptophysin was observed in Mouse Brain in comparison to Mouse Heart and Mouse Liver using Anti-Synaptophysin Monoclonal Antibody (SY38) (Product # MA1-213) in Western Blot. {RE}

Product Details

MA1-213

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:1,500

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:500

Immunohistochemistry (PFA fixed) (IHC (PFA))

Assay-dependent

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:500

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

1:200-1:500

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

SY38

Immunogen

Synaptophysin from presynaptic vesicles from bovine brain

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2723681

Product Specific Information

MA1-213 has been successfully used in western blot, imunofluorescence, immunohistochemistry, flow cytometry, immunoprecipitation and ELISA.

MA1-213 detects Synaptophysin in human, bovine, mouse and rat samples.

Target Information

Synaptophysin is a calcium-binding and integral membrane glycoprotein present in presynaptic vesicles in almost all neurons. Synaptophysin has four transmembrane domains and it forms a complex with dynamin at high calcium concentrations suggesting an involvement in synaptic vesicle endocytosis. It is also involved in the regulation of short-term and long-term synaptic plasticity. Synaptophysin is currently the most widely used marker for nerve terminals and for differentiating neuroendocrine tumors. Mutations in the gene can result in mental retardation, X-linked 96.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Multimodal monitoring of human cortical organoids implanted in mice reveal functional connection with visual cortex.

Nature communications

Wilson MN,Thunemann M,Liu X,Lu Y,Puppo F,Adams JW,Kim JH,Ramezani M,Pizzo DP,Djurovic S,Andreassen OA,Mansour AA,Gage FH,Muotri AR,Devor A,Kuzum D

MA1-213 was used in Immunohistochemistry to combine transparent microelectrode arrays and two-photon imaging for longitudinal, multimodal monitoring of human cortical organoids transplanted into the retrosplenial cortex of adult mice. Two-photon imaging shows vascularization of the transplanted organoid.