NeoBiotechnologies

Synaptophysin Monoclonal Antibody (SYP, 3551)

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Synaptophysin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis performed in formalin fixed, paraffin-embedded human pancreatic carcinoma using Synaptophysin Monoclonal Antibody (Product # 6855-MSM1-P1ABX).

Synaptophysin Antibody in Peptide array (ARRAY) — Analysis of Protein Array containing more than 19,000 full-length human proteins Synaptophysin Monoclonal Antibody (Product # 6855-MSM1-P1ABX). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SDís) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDís) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Synaptophysin Antibody — SDS-PAGE Analyis Purified Synaptophysin Monoclonal Antibody (Product # 6855-MSM1-P1ABX). Confirmation of Purity and Integrity of Antibody.

Product Details

6855-MSM1-P1ABX

Applications	Tested Dilution	Publications
Immunohistochemistry (Paraffin) (IHC (P))	1-2 µg/mL	-
Peptide Array (Array)	Assay-dependent	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Mouse / IgG1, kappa
Class	Monoclonal
Type	Antibody
Clone	SYP, 3551
Immunogen	Recombinant fragment (around aa 224-313) of human Synaptophysin (SYP) protein
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein G
Storage buffer	PBS, pH 7.4
Contains	no preservative
Storage conditions	-20°C or -80°C if preferred

Product Specific Information

Immunohistochemistry (PFA fixed): incubate antibody for 30 min RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degrees C followed by cooling at RT for 20 minutes.

Target Information

Synaptophysin is a calcium-binding and integral membrane glycoprotein present in presynaptic vesicles in almost all neurons. Synaptophysin has four transmembrane domains and it forms a complex with dynamin at high calcium concentrations suggesting an involvement in synaptic vesicle endocytosis. It is also involved in the regulation of short-term and long-term synaptic plasticity. Synaptophysin is currently the most widely used marker for nerve terminals and for differentiating neuroendocrine tumors. Mutations in the gene can result in mental retardation, X-linked 96.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.