Invitrogen

Synaptotagmin 7 Monoclonal Antibody (S275-14)

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Synaptotagmin 7 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of Synaptotagmin 7 in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with Synaptotagmin 7 monoclonal antibody (Product # MA5-27654) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:100, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain, B) Phalloidin Texas Red F-Actin stain, C) Synaptotagmin-7 Antibody, D) Merged image. Magnification: 60x.

Synaptotagmin 7 Antibody in Western Blot (WB) — Western blot analysis of Synaptotagmin 7 in rat brain lysates. The sample was incubated with Synaptotagmin 7 monoclonal antibody (Product # MA5-27654) using the following dilutions: 1:100, 1:250, 1:500 and 1:1000.

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

1:100

Immunofluorescence (IF)

1:100

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:1000

Western Blot (WB)

1:1000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host / Isotype

Mouse / IgG2B

Class

Monoclonal

Type

Antibody

Clone

S275-14

Immunogen

Fusion protein amino acids 150-239 (Cytoplasmic C2A domain) of mouse Synaptotagmin-7

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20°C

RRID

AB_2735383

Target Information

This gene is a member of the synaptotagmin gene family and encodes a protein similar to other family members that mediate calcium-dependent regulation of membrane trafficking in synaptic transmission. A similar protein in rodents mediates hormone secretion and lysosome exocytosis. In humans, expression of this gene has been associated with prostate cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: IPCA-7; PCANAP7; Prostate cancer-associated protein 7; Protein Syt7; Synaptotagmin 7; Synaptotagmin VII; Synaptotagmin-7; SYT-7; SYT7; SytVII

Gene Aliases: AI851541; B230112P13Rik; IPCA-7; IPCA7; PCANAP7; SYT-VII; SYT7; SYTVII

UniProt ID: (Human) O43581, (Mouse) Q9R0N7, (Rat) Q62747

Entrez Gene ID: (Human) 9066, (Mouse) 54525, (Rat) 59267

Molecular Function: membrane traffic protein membrane trafficking regulatory protein