|Tested species reactivity||Bovine, Mouse, Non-human primate, Rat, Shark|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Rat brain synaptic junction protein complexes.|
|Storage buffer||PBS, pH 7.2, with 0.1mM PMSF, 50% glycerol|
|Contains||0.09% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||0.1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-25482 detects Synaptotagmin from rat, bovine, monkey, shark, and mouse samples.
MA1-25482 has been successfully used in immunoprecipitation, immunohistochemistry, immunocytochemistry and Western blot procedures. Possible positive controls for this product are rat brain tissue extract.
The MA1-25482 immunogen is rat brain synaptic junction protein complexes.
Store at 4°C short term. For extended storage, store at -20°C, avoiding freeze/thaw cycles.
The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin participates in triggering neurotransmitter release at the synapse. The first C2 domain mediates Ca(2+)-dependent phospholipid binding. The second C2 domain mediates interaction with Stonin 2. Synaptotagmin may have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.