|Tested species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to amino acid residues specific to rat synaptotagmin 1 conjugated to KLH|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1/1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with human, mouse, bovine, chicken and non-human primate based on 100% sequence homology.
In Western blot, this antibody is specific for ~65 kDa synaptotagmin I protein.
Synaptotagmin 1 is a synaptic vesicle membrane glycoprotein that is widely expressed throughout the CNS and is generally thought to act as the Ca2+ sensor in the regulation of exocytosis and neurotransmitter release (Littleton and Bellen 1995). Recent studies indicate that synaptotagmin and quote;s Ca2+ mediated binding of SNAP25 is essential for the Ca2+ dependent triggering of membrane fusion (Zhang et al., 2002). It has recently been demonstrated that discrete residues within the c(2)b binding domain of synaptotagmin 1 independently specify endocytic rate and synaptic vesicle size (Poskanzer et al., 2006).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.