Immunohistochemistry analysis of Syndecan-1 / CD138 showing staining in the cytoplasm and membrane of paraffin-embedded human small intestinal tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Syndecan-1 / CD138 polyclonal antibody (362900) diluted in 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from an internal region of the human Syndecan-1 protein|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||1:100-1:500|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20-1:200|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
CD138 (syndecan 1) is a transmembrane proteoglycan that can bind a variety of cytokines and modulate their activity, as well as the activity of extracellular matrix components and influence many developmental processes. CD138 is expressed mainly in differentiating keratinocytes and is transiently upregulated in all layers of the epidermis upon tissue injury. It is also highly expressed on plasma cells and can be detected even on fibroblasts, vascular smooth muscle cells and endothelial cells. Up-regulation and down-regulation of CD138 on the cell surface often correlates with the gain of cancerous characteristics. Serum levels of the shedded soluble sCD138 are used as a prognostic factor of cancerogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bmp signaling in colonic mesenchyme regulates stromal microenvironment and protects from polyposis initiation.
36-2900 was used in western blot to determine how Bmp signaling in colonic mesenchyme regulates stromal microenvironment and protects from initiation of polyposis
|Allaire JM,Roy SA,Ouellet C,Lemieux É,Jones C,Paquet M,Boudreau F,Perreault N||International journal of cancer (138:2700)||2016|
|Human||2 µg/ml||Primary human glomerular endothelial cells produce proteoglycans, and puromycin affects their posttranslational modification.||Björnson A,Moses J,Ingemansson A,Haraldsson B,Sörensson J||American journal of physiology. Renal physiology (288:F748)||2005|
|Human||Not Cited||Heparan sulfate proteoglycans are required for cellular binding of the hepatitis E virus ORF2 capsid protein and for viral infection.||Kalia M,Chandra V,Rahman SA,Sehgal D,Jameel S||Journal of virology (83:12714)||2009|