Immunofluorescence analysis of Syndecan-1 / CD138 was performed using 70% confluent log phase COLO 205 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Syndecan-1 / CD138 Rabbit Polyclonal Antibody (Product # PA5-16918) at 2µg/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjµgate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic and nuclear localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein encoding aa 18-218 human CD138|
|Storage buffer||PBS, pH 7.6, with 0.2% BSA|
|Contains||15mM sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 2 publications below|
PA5-16918 targets CD138 in IHC applications and shows reactivity with Human samples.
The PA5-16918 immunogen is recombinant protein encoding aa 18-218 human CD138.
CD138/Syndecan-1 is a transmembrane heparin sulphate proteoglycan which is made up of one core protein and five glycosaminoglycan. CD138 is expected to play a role in cell adhesion. It is expressed on the surface of pre-B cells and plasma cells but is absent from mature B cells. It is a selective marker for B cell lymphoblastic leukemia and lymphoplasmocytoid leukemia. It is lost from the apoptotic myeloma cells; hence is a useful marker for viable myeloma cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Low levels of monoclonal small B cells in the bone marrow of patients with diffuse large B-cell lymphoma of activated B-cell type but not of germinal center B-cell type.
PA5-16918 was used in immunohistochemistry to investigate the presence of monoclonal small B cells on diffuse large B-cell lymphoma
|Tierens AM,Holte H,Warsame A,Ikonomou IM,Wang J,Chan WC,Delabie J||Haematologica (95:1334)||2010|
Proliferation of immature plasma cells in pouchitis mucosa in patients with ulcerative colitis.
PA5-16918 was used in immunohistochemistry to study the abnormal plasma cell proliferation in pouchitis mucosa from patients with ulcerative colitis
|Hirata N,Oshitani N,Kamata N,Sogawa M,Yamagami H,Watanabe K,Watanabe T,Tominaga K,Fujiwara Y,Maeda K,Hirakawa K,Arakawa T||Inflammatory bowel diseases (14:1084)||2008|