|Tested species reactivity||Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant human CD138 protein.|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is tonsil tissue.
CD138 / Syndecan-1, is a transmembrane, heparin sulphate proteoglycan which is made up of one core protein and five glycosaminoglycans. CD138 is expected to play a role in cell adhesion, cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is expressed on the surface of pre-B cells and plasma cells but is absent from mature B cells. It is lost from the apoptotic myeloma cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Mammary serine protease inhibitor and CD138 immunohistochemical expression in ovarian serous and clear cell carcinomas.
MA5-16331 was used in immunohistochemistry - paraffin section to characterize ovarian serous and clear cell carcinomas for CD138 immunohistochemical expression and mammary serine protease inhibitor
|Hasby EA||Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine (37:4889)||2016|