Western blot analysis of Syntrophin alpha 1 in human Muscle lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide using a Syntrophin alpha 1 polyclonal antibody (Product # PA1-32071) at a dilution of 0.01µg/ml following incubation for 1 hour and detected using chemiluminescence.
|Tested species reactivity||Bovine, Dog, Human, Mouse, Pig, Rat|
|Host / Isotype||Goat / IgG|
|Immunogen||Peptide with sequence ASGRRAPRTGLLE, from N Terminus of the protein sequence according to NP_003089|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-32071 detects Syntrophin alpha 1 from human samples.
PA1-32071 has been successfully used in Western blot applications.
The PA1-32071 immunogen is: Peptide with sequence ASGRRAPRTGLLE, from N Terminus of the protein sequence according to NP_003089
Dystrophin is a large, rod-like cytoskeletal protein found at the inner surface of muscle fibers. Dystrophin is missing in Duchenne Muscular Dystrophy patients and is present in reduced amounts in Becker Muscular Dystrophy patients. The protein encoded by this gene is a peripheral membrane protein found associated with dystrophin and dystrophin-related proteins. This gene is a member of the syntrophin gene family, which contains at least two other structurally-related genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.