Invitrogen

Syntrophin alpha-1 Polyclonal Antibody

View all (37) Syntrophin alpha-1 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

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Syntrophin alpha-1 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of Syntrophin alpha-1 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115997) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).

Syntrophin alpha-1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Syntrophin alpha-1 in human colorectal cancer. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-115997) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

Syntrophin alpha-1 Antibody in Western Blot (WB) — Western blot analysis of Syntrophin alpha-1 in A549 cells (left lane: treated with blocking peptide). Samples were incubated with polyclonal antibody (Product # PA5-115997).

Product Details

PA5-115997

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:50-1:200

Immunocytochemistry (ICC/IF)

1:100-1:500

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human SNTA1(Accession Q13424), corresponding to amino acid residues V163-D188.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

RRID

AB_2900631

Target Information

Syntrophin alpha-1 (Dystrophin, SNTA1) is a large, rod-like cytoskeletal protein found at the inner surface of muscle fibers. Dystrophin is missing in Duchenne Muscular Dystrophy patients and is present in reduced amounts in Becker Muscular Dystrophy patients. The protein encoded by this gene is a peripheral membrane protein found associated with dystrophin and dystrophin-related proteins. This gene is a member of the syntrophin gene family, which contains at least two other structurally-related genes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 59 kDa dystrophin-associated protein A1 acidic component 1; acidic alpha 1 syntrophin; Alpha-1-syntrophin; alpha1-syntrophin; dystrophin-associated protein A1, 59kDa, acidic component; Pro-TGF-alpha cytoplasmic domain-interacting protein 1; RP23-192L3.1; syntrophin, acidic 1; syntrophin, alpha 1 (dystrophin-associated protein A1, 59kDa, acidic component); Syntrophin-1; TACIP1

Gene Aliases: AW228934; dJ1187J4.5; LQT12; SNT1; SNTA1; TACIP1

UniProt ID: (Human) Q13424

Entrez Gene ID: (Human) 6640, (Mouse) 20648, (Rat) 362242

Function(s)

regulation of heart rate muscle contraction regulation of ventricular cardiac muscle cell membrane repolarization ventricular cardiac muscle cell action potential negative regulation of peptidyl-cysteine S-nitrosylation regulation of sodium ion transmembrane transport regulation of vasoconstriction by circulating norepinephrine neuromuscular junction development biological_process

Process(es)

actin binding structural molecule activity protein binding calmodulin binding sodium channel regulator activity PDZ domain binding ion channel binding nitric-oxide synthase binding ATPase binding