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Immunofluorescent analysis of Synuclein (green) showing staining in the cytoplasm and membrane of U87-MG cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Synuclein monoclonal antibody (Product # MA5-12272) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Human, Rat|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human recombinant alpha-synuclein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:200-1:400|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-12272 targets Synuclein in IHC (P), ICC/IF, IP, and WB applications and shows reactivity with Human and Rat samples.
The MA5-12272 immunogen is human recombinant alpha-synuclein.
Synnuclein family of proteins is abundantly expressed in neuronal cytosol and presynaptic terminals. In vertebrates they are encoded by three different genes. Synucleins have been specifically implemented in three major diseases: Alzheimer's (AD), Parkinson's PD) and breast cancer. In AD, a peptide derived from alpha-synuclein forms an intrinsic component of plaque amyloid. In PD, an alpha synuclein accumulates in Lewy bodies. An allele of alpha synuclein has been linked to many familial cases of PD. In breast cancer increased expression of gamma synuclein correlates with the disease progression. Synucleins appear to be involved in the membrane plasticity in developing song control system of songbirds.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Differential expression of alpha-synuclein in hippocampal neurons.
MA5-12272 was used in immunocytochemistry to study the hippocampal neuronal expression of alpha-synuclein under normal and pathological conditions
|Taguchi K,Watanabe Y,Tsujimura A,Tatebe H,Miyata S,Tokuda T,Mizuno T,Tanaka M||PloS one (9:null)||2014|
α-Synuclein membrane association is regulated by the Rab3a recycling machinery and presynaptic activity.
MA5-12272 was used in western blot to study the role of Rab3a and synaptic activity in the membrane association of alpha-synuclein
|Chen RH,Wislet-Gendebien S,Samuel F,Visanji NP,Zhang G,Marsilio D,Langman T,Fraser PE,Tandon A||The Journal of biological chemistry (288:7438)||2013|
α-synuclein phosphorylation and truncation are normal events in the adult human brain.
MA5-12272 was used in western blot to study the constitutive truncation and phosphorylation of alpha-synuclein in normal human brain
|Muntané G,Ferrer I,Martinez-Vicente M||Neuroscience (200:106)||2012|
Abnormal alpha-synuclein solubility, aggregation and nitration in the frontal cortex in Pick's disease.
MA5-12272 was used in western blot to study the distinct changes in the solubility, aggregation and tyrosine nitration of alpha-synuclein observed in the frontal cortex in Pick' disease
|Dalfó E,Martinez A,Muntané G,Ferrer I||Neuroscience letters (400:125)||2006|
A reassessment of the neuropathology of frontotemporal dementia linked to chromosome 3.
MA5-12272 was used in immunohistochemistry to re-evaluate the neuropathology of frontotemporal dementia linked to chromosome 3 in a Danish family
|Holm IE,Englund E,Mackenzie IR,Johannsen P,Isaacs AM||Journal of neuropathology and experimental neurology (66:884)||2007|
alpha-synuclein; isoform NACP140; non A-beta component of AD amyloid; non-A beta component of AD amyloid; non-A4 component of amyloid; synuclein alpha-140; synuclein, alpha (non A4 component of amyloid precursor)
NACP; PARK1; PARK4; PD1; SNCA