|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Synthetic peptide to corresponding residues C V(164) A N K S D L Q K T Y Q K L(177) of the human T-catenin protein.|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:25-1:50|
|Immunohistochemistry (Paraffin) (IHC (P))||1:25-1:50|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:100-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-06311 detects alphaT-catenin in human samples.
MA1-06311 has sucessfully been used in Western blotting, immunoprecipitation, immunohistochemistry, and immunocytochemistry. By Western blotting, it detects a ~300 kDa protein representing alphaT-catenin.
The MA1-06311 immunogen is a synthetic peptide corresponding to residues C V(164) A N K S D L Q K T Y Q K L(177) of the human T-catenin protein with N-term Cys, coupled to KLH.
Catenins are proteins that connect cadherin/beta-catenin cell adhesion complexes to the actin cytoskeleton. Although originally discovered in testis, alphaT-catenin is expressed in other tissues, the highest levels being observed in heart. Immunohistochemical analysis showed human alphaTcatenin localization at intercalated discs of cardiomyocytes and in peritubular myoid cells of testis. It is proven that alphaT-catenin can functionally restore cell-cell adhesion in colon cancer cells
lacking alpha-catenins. This indicates that this protein is involved in the formation of specific cell-cell contacts in specific types of muscle cells. The encoded protein has about the same predicted size (100 kDa) as other alpha-catenins to which it shows an overall amino acid identity of 57%. In cells transfected with alphaT-catenin cDNA, interaction with alpha-catenin was demonstrated by coimmunoprecipitation. The 892_24D2S antibody against alphaT-catenin does not show any cross reaction with alphaE-catenin or alphaNcatenin in western blot analysis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.