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| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1:500-1:1,000 | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Recombinant fusion protein containing a sequence corresponding to amino acids 326-535 of human TBX21 |
Conjugate |
Unconjugated |
Form |
liquid |
Concentration |
4.53 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7.3, with 50% glycerol |
Contains |
0.02% sodium azide |
Storage conditions |
-20° C, Avoid Freeze/Thaw Cycles |
RRID |
AB_2806428 |
Immunogen sequence: ENFESMYTSV DTSIPSPPGP NCQFLGGDHY SPLLPNQYPV PSRFYPDLPG QAKDVVPQAY WLGAPRDHSY EAEFRAVSMK PAFLPSAPGP TMSYYRGQEV LAPGAGWPVA PQYPPKMGPA SWFRPMRTLP MEPGPGGSEG RGPEDQGPPL VWTEIAPIRP ESSDSGLGEG DSKRRRVSPY PSSGDSSSPA GAPSPFDKEA EGQFYNYFPN; Positive Samples: B cells; Cellular Location: Nucleus
T-bet, also known as TBX21, is a T-cell specific transcription factor containing a single T-box DNA-binding domain. It controls the expression of the TH1 cytokine, interferon-gamma. Both Th1 and Th17 cells are crucial in immune regulation and autoimmune disease development. T-bet initiates Th1 lineage development from naive Th precursor cells and directs T cell differentiation to Th1 versus Th17. T-bet is cooperating with Stat4 (signal tranducer and activator of transcription 4) in programming chromatin architecture for Th1 gene expression. Genetic variations in T-bet are associated with susceptibility to aspirin-induced asthma and nasal polyps.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: HGNC:11599; T-box expressed in T cells; T-box protein 21; T-box transcription factor TBX21; T-cell-specific T-box transcription factor T-bet; Transcription factor TBLYM
Gene Aliases: T-bet; T-PET; TBET; TBLYM; TBX21
UniProt ID: (Human) Q9UL17
Entrez Gene ID: (Human) 30009
Molecular Function:
Rel homology transcription factor
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