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TAB3 Antibody (MA5-38022) in WB
Western blot analysis of TAB3 in extracts of various cell lines. Samples were incubated with TAB3 Monoclonal antibody (Product # MA5-38022) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Product Details
MA5-38022
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
ARC2350
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TAB3 (Q8N5C8)
Conjugate
Unconjugated
Form
Liquid
Concentration
0.52 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2897940
Product Specific Information
Positive Samples: HeLa, 293T, HepG2, Mouse liver
Immunogen sequence: MAQSSPQLDI QVLHDLRQRF PEIPEGVVSQ CMLQNNNNLE ACCRALSQES SKYLYMEYHS PDDNRMNRNR LLHINLGIHS PSSYHPGDGA QLNGGRTLVH
Target Information
TAB3 functions in the NF-kappaB signal transduction pathway. It and the similar and functionally redundant protein TAB2, form a ternary complex with the protein kinase TAK1 and either TRAF2 or TRAF6 in response to stimulation with the pro-inflammatory cytokines TNF or IL-1. Subsequent TAK1 kinase activity triggers a signaling cascade leading to activation of the NF-kappaB transcription factor. Recent experiments have shown that TAB2 and the related protein TAB3 constitutitvely interact with the autophagy mediator Beclin-1; upon induction of autophagy, these proteins dissociate from Beclin-1 and bind TAK1. Overexpression of TAB2 and TAB3 inhibit autophagy, while their depletion triggers it, suggesting that TAB2 and TAB3 act as a control point for autophagy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: mitogen activated protein kinase kinase kinase 7 interacting protein 3; mitogen-activated protein kinase kinase kinase 7 interacting protein 3; Mitogen-activated protein kinase kinase kinase 7-interacting protein 3; NF-kappa-B-activating protein 1; NFkB activating protein 1; TAB-3; TAK1-binding protein 3; TGF-beta-activated kinase 1 and MAP3K7-binding protein 3; TGF-beta-activated kinase 1-binding protein 3
Gene Aliases: 4921526G09Rik; Kiaa4135; MAP3K7IP3; mKIAA4135; NAP1; TAB3
UniProt ID: (Human) Q8N5C8, (Mouse) Q571K4
Entrez Gene ID: (Human) 257397, (Mouse) 66724
activation of MAPK activity
stimulatory C-type lectin receptor signaling pathway
MyD88-dependent toll-like receptor signaling pathway
I-kappaB kinase/NF-kappaB signaling
JNK cascade
negative regulation of autophagy
Fc-epsilon receptor signaling pathway
positive regulation of NF-kappaB transcription factor activity
nucleotide-binding oligomerization domain containing signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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