|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Goat / IgG|
|Immunogen||synthetic peptide LQRQNRVDSKETEC (Human), C terminus.|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-31074 detects TACE from human, mouse and rat samples. Expected to cross-react with Chinese Hamster due to sequence homology.
PA1-31074 has been successfully used in Western blot and peptide-ELISA applications. Detects a band of approximately 125-130 kDa (predicted molecular weight: 95 or when glycosylated 130 kDa).
The PA1-31074 immunogen is: Synthetic peptide: LQRQNRVDSKETEC (Human), C terminus.
TNF alpha is a pro-inflammatory cytokine and contributes to a variety of inflammatory disease responses and programmed cell death. It is synthesized as a 26K type II membrane-bound precursor that is cleaved by aconvertase to generate secreted 17K mature TNF alpha. TNF alpha converting enzyme (TACE) protein was recently purified and the human and mouse TACE cDNAs were cloned by several groups separately. TACE is a membrane-bound metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease). TACE also processes other cell surface proteins, including TNF receptor, TGF alpha, the L-selectin adhesion molecule, and alpha-cleavage of amyloid protein precursor (APP). TACE mRNA is expressed in a variety of human and murine tissues. TACE was selected as one of the few targets in cytokine activation by the Eighth International Conference of the Inflammation Research Association.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.