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|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to amino acids 380-432 of human TAF II p100|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.2|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:200|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody detects endogenous protein at a molecular weight of 87 kDa.
Purity is >95% by SDS-PAGE.
TFIID is a general transcription factor which initiates preinitiation complex assembly through direct interaction with the TATA promoter element. It is a multisubunit complex consisting of a small TATA-binding polypeptide andother TATA-binding protein (TBP)-associated factors (TAFs). Although native TFIID can mediate both activator-independent (basal) and activator-dependent transcription in reconstituted systems, TBP can mediate only basal transcription. TAF II p100 (TBP-associated factor II100), also known as TAF5 or TAFII100, is the third largest subunit of human TFIID. It contains six WD40 repeats at the C-terminus and has an N-terminus capable of forming a flexible dimer. TAF II p100 plays an important role in forming the scaffold that is crucial for the assembly of the TFIID complex. TAF II p100 may also be involved in the stabilization of TAF interactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
TAF(II)100; TAF2D; TAF5; TAF5 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 100kDa; TAFII-100; TAFII100; TATA box binding protein (TBP)-associated factor 2D; TATA box binding protein (TBP)-associated factor, RNA polymerase II, D, 100kD; TATA box binding protein associated factor 5; Tbp-associated factor 5; transcription initiation factor TFIID 100 kD subunit; transcription initiation factor TFIID 100 kDa subunit; transcription initiation factor TFIID subunit 5
6330528C20Rik; AV117817; TAF(II)100; TAF2D; TAF5; TAFII-100; TAFII100