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|Tested species reactivity||Yeast|
|Host / Isotype||Rabbit / IgG|
|Immunogen||the N-terminal domain of Taf4p protein (amino acid residues 1-200)|
|Storage buffer||whole serum|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Western Blot (WB)||1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-4936 detects Taf4p in budding yeast samples.
PA1-4936 has been successfully used in Western blot and ELISA procedures. By Western blot this antibody detects a ~48 kDa protein (Taf4p).
PA1-4936 immunogen corresponds to the recombinant protein which was over-expressed in E. coli with a plasmid carrying the N-terminal domain of Taf4p protein (amino acid residues 1-200).
The basal transcription factor TFIID plays a central role in the regulation of gene expression in Eukaryota and is a large protein complex composed of TATA box-binding protein (TBP) and 14 kinds of TBP-associated factors (TAF). TFIID directly recognizes and binds to different kinds of core promoter elements that localize near the transcription initiation site and forms a scaffold for the other basal transcription factors to assemble. At the same time, it transmits transcriptional activation signal originating from transcription regulating factors to RNA polymerase II. Taf4p is one of the subunits of TFIID and in the case of budding yeast, it is composed of 388 amino acid residues (aa). This protein contains histone folds in its interior and forms TAF octamer with Taf6p, Taf9p and Taf12p.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.