MA3-077 detects TATA Binding protein (TBP) Associated factor 6 in human and mouse samples.
MA3-077 has been successfully used in a Western blot application.
The MA3-077 immunogen is a synthetic peptide coupled to ovalalbumin, from aa 39 to aa 56.
Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the smaller subunits of TFIID that binds weakly to TBP but strongly to TAF1, the largest subunit of TFIID. Four isoforms have been identified but complete transcripts have been determined for only three isoforms. One of the isoforms has been shown to preclude binding of one of the other TFIID subunits, thereby reducing transcription and initiating signals that trigger apoptosis.
Protein Aliases: DKFZp781E21155; p80; RNA polymerase II TBP-associated factor subunit E; TAF(II)70; TAF(II)80; TAF6 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 80 kDa; TAF6 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 80kDa; TAFII-70; TAFII-80; TAFII80; TATA box binding protein (Tbp)-associated factor, RNA polymerase II, E; TATA box binding protein (TBP)-associated factor, RNA polymerase II, E, 70/85kD; TATA box binding protein associated factor 6; Transcription initiation factor TFIID 70 kDa subunit; Transcription initiation factor TFIID 80 kDa subunit; Transcription initiation factor TFIID subunit 6
Gene Aliases: 80kDa; AW549759; MGC:8964; p80; TAF(II)70; TAF(II)80; TAF2E; TAF6; TAFII-70; TAFII-80; TAFII70; TAFII80; TAFII85
Molecular Function: general transcription factor