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| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1:500-1:2,000 | - |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:150 | - |
Immunocytochemistry (ICC/IF) |
1:100 | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human, Mouse |
Host/Isotype |
Mouse / IgG1 |
Class |
Monoclonal |
Type |
Antibody |
Clone |
OTI8C8 |
Immunogen |
Full length human recombinant protein of human TAGLN produced in HEK293T cell. |
Conjugate |
Unconjugated |
Form |
lyophilized |
Concentration |
1 mg/mL |
Purification |
Affinity chromatography |
Storage buffer |
PBS, pH 7.3, with 8% trehalose |
Contains |
no preservative |
Storage conditions |
-20° C, Avoid Freeze/Thaw Cycles |
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
TAGLN is a transformation and shape-change sensitive actin cross-linking/gelling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 22 kDa actin-binding protein; Actin-associated protein p27; DKFZp686B01212; DKFZp686P11128; Protein WS3-10; sm22 alpha; SM22-alpha; Sm22alpha; Smooth muscle protein 22-alpha; Transgelin; transgelin variant 2
Gene Aliases: SM22; Sm22a; SMCC; TAGLN; TAGLN1; WS3-10; Ws310
UniProt ID: (Human) Q01995, (Mouse) P37804
Entrez Gene ID: (Human) 6876, (Mouse) 21345
Molecular Function:
non-motor actin binding protein
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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