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Fluorescent Quenching analysis was performed by loading 1µg of purified TAMRA-conjugated BSA (T-BSA) or BSA alone (BSA) into a 96 well plate. Protein was then bound to 10 µg of either Mouse IgG whole molecule (Product # 31202) or quenching antibody Anti-TAMRA clone # 11H10 (Product # MA1-042) and compared to unbound TAMRA-BSA. Emissions were read using the Tecan Safire at 550 nm excitation wavelength and reported in RFU (relative fluorescent units).
|Tested species reactivity||Chemical, Tag|
|Host / Isotype||Mouse / IgG|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Fluorescent Quenching (FQ)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-042 has been successfully used in fluorescence quenching applications.
The red-fluorescent tetramethylrhodamine (TAMRA) azide can be used as a reactive-azido group (Staudinger reagents) that facilitates multiplex labeling when used with phosphine- or alkyne-derivatized tags. In addition, TAMRA fluorophore has been used to label and detect active serine hydrolases in samples by fluorescent gel imaging, capillary electrophoresis or mass spectrometry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.