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Western blot analysis was performed by loading various amounts of purified TAMRA-BSA protein onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were then probed with a mouse monoclonal antibody recognizing TAMRA (Product #MA1-041) at a dilution of 1:1000 for 1hour at room temperature on a rocking platform. Membranes were then washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody (Product #31430) at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection was performed using Pierce Super Signal West Pico (Product #34087).
|Tested species reactivity||Chem, Tag|
|Published species reactivity||Tag|
|Host / Isotype||Mouse / IgG|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||10 µg|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunoprecipitation (IP)||See 1 publications below|
MA1-041 detects TAMRA in samples and has been used in western blotting and immunoprecipitation applications.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
High-resolution functional proteomics by active-site peptide profiling.
MA1-041 was used in immunoprecipitation to enrich for TAMRA-labeled peptides and then eluted and analyzed LC-MS/MS.
|Okerberg ES,Wu J,Zhang B,Samii B,Blackford K,Winn DT,Shreder KR,Burbaum JJ,Patricelli MP||Proceedings of the National Academy of Sciences of the United States of America (102:4996)||2005|