|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic peptide corresponding to the N-terminal residues 1-20 of human, mouse, and rat TBP.|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Gel Shift (GS)||Assay dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 3 publications below|
MA1-21516 detects TATA binding protein (TBP) in human, mouse, and rat samples. This antibody does not cross-react with Drosophila melanogaster, S. cerevisiae, silk worm, or Xenopus laevis.
MA1-21516 has been successfully used in gel shift, immunoprecipitation, and Western blot procedures.
The MA1-21516 immunogen is a synthetic peptide corresponding to the N-terminal residues 1-20 of human, mouse, and rat TBP.
Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. A distinctive feature of TBP is a long string of glutamines in the N-terminal. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. Mutations that expand the number of CAG repeats encoding this polyglutamine tract, and thus increase the length of the polyglutamine string, are associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Loss of Runx2 sensitises osteosarcoma to chemotherapy-induced apoptosis.
MA1-21516 was used in western blot to determine the sensitization of osteosarcoma to chemotherapy-induced apoptosis by a loss of Runx2
|Roos A,Satterfield L,Zhao S,Fuja D,Shuck R,Hicks MJ,Donehower LA,Yustein JT||British journal of cancer (113:1289)||2015|
The RIP140 gene is a transcriptional target of E2F1.
MA1-21516 was used in western blot to study the role of the RIP140 gene as a new transcriptional target of E2F1
|Docquier A,Augereau P,Lapierre M,Harmand PO,Badia E,Annicotte JS,Fajas L,Cavaillès V||PloS one (7:null)||2012|
Wild-type p53-induced phosphatase 1 dephosphorylates histone variant gamma-H2AX and suppresses DNA double strand break repair.
MA1-21516 was used in western blot to investigate the regulation of gamma-H2AX's phosphorylation status by WIP1 and its relationship with the repair of double-stranded DNA damage
|Moon SH,Lin L,Zhang X,Nguyen TA,Darlington Y,Waldman AS,Lu X,Donehower LA||The Journal of biological chemistry (285:12935)||2010|