|ChIP assay (ChIP)||5-10 µg for 25 µg of chromatin|
|Flow Cytometry (Flow)||Assay dependent|
|Immunocytochemistry (ICC)||1 µg/ml|
|Immunofluorescence (IF)||1 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay dependent|
|Western Blot (WB)||1:500-1:2500|
|Western Blot (WB)||See 2 publications below|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Tested Species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human , Mouse|
|Host / Isotype||Mouse / IgG2a, kappa|
|Immunogen||Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human TATA binding protein TBP.|
|Storage buffer||PBS, pH 7.4, with 0.4M arginine|
|Contains||0.02% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
Heat mediated antigen retrieval recommended prior to tissue staining.
This antibody is predicted to react with chicken, cow, Xenopus laevis, chimpanzee and zebrafish based on sequence homology.
Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors to facilitate complex assembly and transcription initiation. This gene encodes TBP, the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-terminus. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. The number of CAG repeats encoding the polyglutamine tract is usually 32-39, and expansion of the number of repeats increases the length of the polyglutamine string and is associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease. Two transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: RP1-191N21.3, GTF2D, GTF2D1, HDL4, MGC117320, MGC126054, MGC126055, SCA17, TFIID, TATA sequence-binding protein; TATA box binding protein; TATA sequence-binding protein; TATA-binding factor; TATA-binding protein; TATA-box binding protein N-terminal domain; TATA-box factor; TATA-box-binding protein; TFIID core protein; Transcription initiation factor TFIID TBP subunit
Gene Aliases: GTF2D; GTF2D1; HDL4; SCA17; TBP; TF2D; TFIID
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