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Immunohistochemical (formalin-fixed, paraffin-embedded) staining of Human Breast Carcinoma tissue using MA5-14739, anti-TATA binding protein TBP antibody. Primary antibody was used at a concentration of 1 ug/ml and exposed for 15 mins at room temp. The sample was pretreated using heat mediated antigen retrieval with Sodium Citrate Buffer (pH6 / 20mins). The detection method was a HRP conjugated polymer, DAB chromogen and the sample was counterstained with haematoxylin and mounted with DPX.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Mouse|
|Host / Isotype||Mouse / IgG2a, kappa|
|Immunogen||Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human TATA binding protein TBP.|
|Storage buffer||PBS, pH 7.4, with 0.4M arginine|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||5-10 µg for 25 µg of chromatin|
|Flow Cytometry (Flow)||Assay dependent|
|Immunocytochemistry (ICC)||1 µg/ml|
|Immunofluorescence (IF)||1 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay dependent|
|Western Blot (WB)||1:500-1:2500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
Heat mediated antigen retrieval recommended prior to tissue staining.
This antibody is predicted to react with chicken, cow, Xenopus laevis, chimpanzee and zebrafish based on sequence homology.
Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors to facilitate complex assembly and transcription initiation. This gene encodes TBP, the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-terminus. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. The number of CAG repeats encoding the polyglutamine tract is usually 32-39, and expansion of the number of repeats increases the length of the polyglutamine string and is associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease. Two transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
mTORC1 Down-Regulates Cyclin-Dependent Kinase 8 (CDK8) and Cyclin C (CycC).
MA5-14739 was used in western blot to demonstrate that mTORC1 activation downregulates the CDK8-CycC complex and elevates lipogenic protein expression.
|Feng D,Youn DY,Zhao X,Gao Y,Quinn WJ,Xiaoli AM,Sun Y,Birnbaum MJ,Pessin JE,Yang F||PloS one (10:null)||2015|
RP1-191N21.3, GTF2D, GTF2D1, HDL4, MGC117320, MGC126054, MGC126055, SCA17, TFIID, TATA sequence-binding protein; TATA box binding protein; TATA sequence-binding protein; TATA-binding factor; TATA-binding protein; TATA-box binding protein N-terminal domain; TATA-box factor; TATA-box-binding protein; TFIID core protein; transcription initiation factor TFIID TBP subunit
GTF2D; GTF2D1; HDL4; SCA17; TBP; TF2D; TFIID