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Immunofluorescence analysis of TAU was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with TAU Rabbit Polyclonal Antibody (PA516380) at 2 ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein encoding C-terminal part of human tau|
|Storage buffer||whole serum diluted in PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2-3 µg/ml|
|Immunofluorescence (IF)||2-3 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-16380 targets TAU in IHC (P) applications and shows reactivity with Human samples.
The PA5-16380 immunogen is recombinant protein encoding C-terminal part of human tau.
Tau proteins are microtubule-associated proteins (MAPs). Alternate splicing of tau mRNA and differential phosphorylation contribute to the heterogeneity of tau.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Fluorescent rhodanine-3-acetic acids visualize neurofibrillary tangles in Alzheimer's disease brains.
PA5-16380 was used in immunohistochemistry to study the detection of neurofibrilliary tangles in Alzheimer's disease brains using fluorescent rhodamine-3-acetic acid-based imaging probes
|Anumala UR,Gu J,Lo Monte F,Kramer T,Heyny-von Haußen R,Hölzer J,Goetschy-Meyer V,Schön C,Mall G,Hilger I,Czech C,Herms J,Schmidt B||Bioorganic & medicinal chemistry (21:5139)||2013|
Design, synthesis and biological evaluation of trimethine cyanine dyes as fluorescent probes for the detection of tau fibrils in Alzheimer's disease brain and olfactory epithelium.
PA5-16380 was used in immunohistochemistry to study the use of fluorescent trimethine cyanine dyes for the detection of tau fibrils in postmortem Alzheimer's disease brain
|Gu J,Anumala UR,Heyny-von Haußen R,Hölzer J,Goetschy-Meyer V,Mall G,Hilger I,Czech C,Schmidt B||ChemMedChem (8:891)||2013|
Bis(arylvinyl)pyrazines, -pyrimidines, and -pyridazines as imaging agents for tau fibrils and β-amyloid plaques in Alzheimer's disease models.
PA5-16380 was used in immunohistochemistry to investigate the potential of fluorescent pyrazine, pyrimidine, and pyridazine derivatives for tau-based diagnosis of Alzhemer's disease
|Boländer A,Kieser D,Voss C,Bauer S,Schön C,Burgold S,Bittner T,Hölzer J,Heyny-von Haußen R,Mall G,Goetschy V,Czech C,Knust H,Berger R,Herms J,Hilger I,Schmidt B||Journal of medicinal chemistry (55:9170)||2012|
Molecular mechanism of tau aggregation induced by anionic and cationic dyes.
PA5-16380 was used in immunocytochemistry and western blot to study the molecular mechanism by which anionic dyes promote tau aggregation and the inhibitory mechanism of the cationic dye methylene blue
|Lira-De León KI,García-Gutiérrez P,Serratos IN,Palomera-Cárdenas M,Figueroa-Corona Mdel P,Campos-Peña V,Meraz-Ríos MA||Journal of Alzheimer's disease : JAD (35:319)||2013|
G protein beta1/gamma2 subunit-interacting factor 1; isoform 4; microtubule-associated protein tau; microtubule-associated protein tau, isoform 4; neurofibrillary tangle protein; paired helical filament-tau; PHF-tau; protein phosphatase 1, regulatory subunit 103
DDPAC; FTDP-17; MAPT; MAPTL; MSTD; MTBT1; MTBT2; PPND; PPP1R103; TAU