Immunofluorescence analysis of TAU was done on 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with TAU (TAU-5) Mouse Monoclonal Antibody (MA512808) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Bovine, Human, Mouse, Sheep, Rat|
|Published species reactivity||Rat, Mouse, Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified bovine microtubule-associated proteins|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||2-3 µg/mL|
|Immunofluorescence (IF)||2-3 µg/mL|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Immunoprecipitation (IP)||2µg/mg protein lysate|
|Western Blot (WB)||1-2 µg/ml|
MA5-12808 targets TAU in immunofluorescence, immunoprecipitation, and Western blot applications and shows reactivity with Bovine, Human, mouse, Ovine, and Rat samples.
The MA5-12808 immunogen is purified bovine microtubule-associated proteins.
Tau proteins are microtubule-associated proteins (MAPs). Alternate splicing of tau mRNA and differential phosphorylation contribute to the heterogeneity of tau.
Tau reduction prevents disease in a mouse model of Dravet syndrome.
MA5-12808 was used in immunohistochemistry to assess if tau reduction benefits intractable genetic epilepsies
|Gheyara AL,Ponnusamy R,Djukic B,Craft RJ,Ho K,Guo W,Finucane MM,Sanchez PE,Mucke L||Annals of neurology (76:443)||2014|
Activation of sirtuin 1 attenuates cerebral ventricular streptozotocin-induced tau hyperphosphorylation and cognitive injuries in rat hippocampi.
MA5-12808 was used in western blot to study the protective effects of pharmacological SIRT1 activation against tau hyperphosphorylation and memory deficits in a rat model of brain insulin resistance
|Du LL,Xie JZ,Cheng XS,Li XH,Kong FL,Jiang X,Ma ZW,Wang JZ,Chen C,Zhou XW||Age (Dordrecht, Netherlands) (36:613)||2014|
A protocol for concurrent high-quality immunohistochemical and biochemical analyses in adult mouse central nervous system.
MA5-12808 was used in western blot to identify a method for simultaneous use of both biochemistry and immunohistochemistry in regards to the same tissue
|Notter T,Panzanelli P,Pfister S,Mircsof D,Fritschy JM||The European journal of neuroscience (39:165)||2014|
Ser9 phosphorylation causes cytoplasmic detention of I2PP2A/SET in Alzheimer disease.
MA5-12808 was used in western blot to study the role of CKII-mediated Ser9 phosphorylation in the cytoplasmic retention of SET in Alzheimer's disease
|Yu G,Yan T,Feng Y,Liu X,Xia Y,Luo H,Wang JZ,Wang X||Neurobiology of aging (34:1748)||2013|
Glycation exacerbates the neuronal toxicity of β-amyloid.
MA5-12808 was used in western blot to study the increased neuronal toxicity of glycated beta-amyloid
|Li XH,Du LL,Cheng XS,Jiang X,Zhang Y,Lv BL,Liu R,Wang JZ,Zhou XW||Cell death & disease (4:null)||2013|
Nmnat2 attenuates Tau phosphorylation through activation of PP2A.
MA5-12808 was used in western blot to study the ability of Nmnat2 overexpression to activate PP2A and reduce levels of tau phosphorylation
|Cheng XS,Zhao KP,Jiang X,Du LL,Li XH,Ma ZW,Yao J,Luo Y,Duan DX,Wang JZ,Zhou XW||Journal of Alzheimer's disease : JAD (36:185)||2013|
NMDA receptor subunit composition determines beta-amyloid-induced neurodegeneration and synaptic loss.
MA5-12808 was used in western blot to study the effect of the subunit composition of NMDA receptors on neurodegeneration and synaptic loss induced by beta-amyloid
|Tackenberg C,Grinschgl S,Trutzel A,Santuccione AC,Frey MC,Konietzko U,Grimm J,Brandt R,Nitsch RM||Cell death & disease (4:null)||2013|
Alzheimer's disease related markers, cellular toxicity and behavioral deficits induced six weeks after oligomeric amyloid-β peptide injection in rats.
MA5-12808 was used in western blot to study the ability of a single intracerebroventricular injection of soluble oligomers of a beta-amyloid fragment to induce Alzhiemers's disease pathology in a rat model
|Zussy C,Brureau A,Keller E,Marchal S,Blayo C,Delair B,Ixart G,Maurice T,Givalois L||PloS one (8:null)||2013|
Overexpression of RCAN1 isoform 4 in mouse neurons leads to a moderate behavioral impairment.
MA5-12808 was used in western blot to study the effect of transgenic overexpression of human RCAN1 on cognitive performance in young and adult mice
|Bhoiwala DL,Koleilat I,Qian J,Beyer B,Hushmendy SF,Mathew A,Bhoiwala DL,Ferland RJ,Crawford DR||Neurological research (35:79)||2013|
Methylglyoxal induces tau hyperphosphorylation via promoting AGEs formation.
MA5-12808 was used in western blot to study the mechanism by which methylglyoxal induces tau hyperphosphorylation
|Li XH,Xie JZ,Jiang X,Lv BL,Cheng XS,Du LL,Zhang JY,Wang JZ,Zhou XW||Neuromolecular medicine (14:338)||2012|
Enriched environment prevents cognitive impairment and tau hyperphosphorylation after chronic cerebral hypoperfusion.
MA5-12808 was used in western blot to study the effect of an enriched environment on cognitive impairment and tau hyperphosphorylation in chronic cerebral hypoperfusion
|Yao ZH,Zhang JJ,Xie XF||Current neurovascular research (9:176)||2012|
Tumor necrosis factor-α synthesis inhibitor 3,6'-dithiothalidomide attenuates markers of inflammation, Alzheimer pathology and behavioral deficits in animal models of neuroinflammation and Alzheimer's disease.
MA5-12808 was used in western blot to study the effects of an inhibitor of TNF-alpha production in animal models of neuroinflammation and Alzheimer's disease
|Tweedie D,Ferguson RA,Fishman K,Frankola KA,Van Praag H,Holloway HW,Luo W,Li Y,Caracciolo L,Russo I,Barlati S,Ray B,Lahiri DK,Bosetti F,Greig NH,Rosi S||Journal of neuroinflammation (9:null)||2012|
AGEs induce Alzheimer-like tau pathology and memory deficit via RAGE-mediated GSK-3 activation.
MA5-12808 was used in western blot to study the mechanism by which advanced glycation end-products induce tau hyperphosphorylation and memory impairment
|Li XH,Lv BL,Xie JZ,Liu J,Zhou XW,Wang JZ||Neurobiology of aging (33:1400)||2012|
A proteomic analysis of MCLR-induced neurotoxicity: implications for Alzheimer's disease.
MA5-12808 was used in western blot to study MCLR-induced neurotoxicity using proteomics
|Li G,Cai F,Yan W,Li C,Wang J||Toxicological sciences : an official journal of the Society of Toxicology (127:485)||2012|
Folate/vitamin-B12 prevents chronic hyperhomocysteinemia-induced tau hyperphosphorylation and memory deficits in aged rats.
MA5-12808 was used in western blot to study the role of vitamin B12 in preventing tau phosphorylation and cognition decline in aged rats
|Wei W,Liu YH,Zhang CE,Wang Q,Wei Z,Mousseau DD,Wang JZ,Tian Q,Liu GP||Journal of Alzheimer's disease : JAD (27:639)||2011|
AMPK activation inhibits apoptosis and tau hyperphosphorylation mediated by palmitate in SH-SY5Y cells.
MA5-12808 was used in western blot to study the role of AMPK activation in inhibiting palmitate-induded apoptosis and tau hyperphosphorylation in SH-SY5Y cells
|Kim J,Park YJ,Jang Y,Kwon YH||Brain research (1418:42)||2011|
Combined expression of tau and the Harlequin mouse mutation leads to increased mitochondrial dysfunction, tau pathology and neurodegeneration.
MA5-12808 was used in western blot to study the interaction between mitochondrial dysfunction and tau pathology in a novel mouse model
|Kulic L,Wollmer MA,Rhein V,Pagani L,Kuehnle K,Cattepoel S,Tracy J,Eckert A,Nitsch RM||Neurobiology of aging (32:1827)||2011|
Altered phosphorylation but no neurodegeneration in a mouse model of tau hyperphosphorylation.
MA5-12808 was used in western blot to study the role of hyperphosphorylation of tau in Alzheimer's disease using a novel transgenic mouse model
|Hundelt M,Fath T,Selle K,Oesterwind K,Jordan J,Schultz C,Götz J,von Engelhardt J,Monyer H,Lewejohann L,Sachser N,Bakota L,Brandt R||Neurobiology of aging (32:991)||2011|
Berberine attenuates calyculin A-induced cytotoxicity and Tau hyperphosphorylation in HEK293 cells.
MA5-12808 was used in western blot to study the mechanism by which berberine attenuates calyculin A-induced cytotoxicity and Tau hyperphosphorylation
|Yu G,Li Y,Tian Q,Liu R,Wang Q,Wang JZ,Wang X||Journal of Alzheimer's disease : JAD (24:525)||2011|
Fuzhisan, a Chinese herbal medicine, inhibits beta-amyloid-induced neurotoxicity and tau phosphorylation through calpain/Cdk5 pathway in cultured cortical neurons.
MA5-12808 was used in western blot to study the mechanism by which a Chinese herbal medicine inhibits beta-amyloid-induced neurotoxicity and tau phosphorylation
|Zhang Z,Zhao R,Tang Y,Wen S,Wang D,Qi J||Neurochemical research (36:801)||2011|
LiCl attenuates thapsigargin-induced tau hyperphosphorylation by inhibiting GSK-3β in vivo and in vitro.
MA5-12808 was used in western blot to investigate the influence of endoplasmic reticulum stress on tau phosphorylation
|Fu ZQ,Yang Y,Song J,Jiang Q,Lin ZC,Wang Q,Zhu LQ,Wang JZ,Tian Q||Journal of Alzheimer's disease : JAD (21:1107)||2011|
Mapping O-GlcNAc modification sites on tau and generation of a site-specific O-GlcNAc tau antibody.
MA5-12808 was used in western blot to investigate the glycosylation of tau protein
|Yuzwa SA,Yadav AK,Skorobogatko Y,Clark T,Vosseller K,Vocadlo DJ||Amino acids (40:857)||2011|
Protective effect of isoflavones against homocysteine-mediated neuronal degeneration in SH-SY5Y cells.
MA5-12808 was used in western blot to study the mechanism for the protective effect of isoflavones on honmocysteine-mediated neuronal degeneration in SH-SY5Y cells
|Park YJ,Jang Y,Kwon YH||Amino acids (39:785)||2010|
Tau dephosphorylation potentiates apoptosis by mechanisms involving a failed dephosphorylation/activation of Bcl-2.
MA5-12808 was used in western blot to study the mechanism by which tau dephosphorylation facilitates apoptosis
|Liu XA,Liao K,Liu R,Wang HH,Zhang Y,Zhang Q,Wang Q,Li HL,Tian Q,Wang JZ||Journal of Alzheimer's disease : JAD (19:953)||2010|
Isoflavones prevent endoplasmic reticulum stress-mediated neuronal degeneration by inhibiting tau hyperphosphorylation in SH-SY5Y cells.
MA5-12808 was used in western blot to investigate the effect of isoflavone on tau phosphorylation in neurons
|Park YJ,Jang YM,Kwon YH||Journal of medicinal food (12:528)||2009|
Inhibition of autophagy causes tau proteolysis by activating calpain in rat brain.
MA5-12808 was used in western blot to study the mechanism by which inhibition of autophagy promotes proteolysis of tau in rat brain
|Zhang JY,Peng C,Shi H,Wang S,Wang Q,Wang JZ||Journal of Alzheimer's disease : JAD (16:39)||2009|
Estradiol attenuates tau hyperphosphorylation induced by upregulation of protein kinase-A.
MA5-12808 was used in western blot to study the effect of estradiol on PKA-induced tau441 hyperphosphorylation in stably transfected HEK293 cells
|Liu XA,Zhu LQ,Zhang Q,Shi HR,Wang SH,Wang Q,Wang JZ||Neurochemical research (33:1811)||2008|
17beta-estradiol attenuates glycogen synthase kinase-3beta activation and tau hyperphosphorylation in Akt-independent manner.
MA5-12808 was used in western blot to study the role of Akt in the attenuation of GSK-3beta activity and tau hyperphosphorylation by 17beta-estradiol
|Shi HR,Zhu LQ,Wang SH,Liu XA,Tian Q,Zhang Q,Wang Q,Wang JZ||Journal of neural transmission (Vienna, Austria : 1996) (115:879)||2008|
Effects of tau phosphorylation on proteasome activity.
MA5-12808 was used in western blot to study the effects of tau phosphorylation on proteasome activity
|Ren QG,Liao XM,Chen XQ,Liu GP,Wang JZ||FEBS letters (581:1521)||2007|
The involvement of glycogen synthase kinase-3 and protein phosphatase-2A in lactacystin-induced tau accumulation.
MA5-12808 was used in western blot to study the involvement of GSK-3 and protein phosphatase-2A in lactacystin-induced tau accumulation
|Ren QG,Liao XM,Wang ZF,Qu ZS,Wang JZ||FEBS letters (580:2503)||2006|
The marijuana component cannabidiol inhibits beta-amyloid-induced tau protein hyperphosphorylation through Wnt/beta-catenin pathway rescue in PC12 cells.
MA5-12808 was used in western blot to investigate the mechanism of tau phosphorylation regulation by cannabidiol in PC12 cells
|Esposito G,De Filippis D,Carnuccio R,Izzo AA,Iuvone T||Journal of molecular medicine (Berlin, Germany) (84:253)||2006|
EWS-FLI1 fusion protein up-regulates critical genes in neural crest development and is responsible for the observed phenotype of Ewing's family of tumors.
MA5-12808 was used in western blot to study whether EWS-FLI1 fusion gene expression is responsible for the primitive neuroectodermal phenotype of Ewing's family tumors
|Hu-Lieskovan S,Zhang J,Wu L,Shimada H,Schofield DE,Triche TJ||Cancer research (65:4633)||2005|
Nitration and oligomerization of tau induced by peroxynitrite inhibit its microtubule-binding activity.
MA5-12808 was used in western blot to study the potential role of tau nitration in Alzheimer's pathology
|Zhang YJ,Xu YF,Chen XQ,Wang XC,Wang JZ||FEBS letters (579:2421)||2005|
Partial rescue of the p35-/- brain phenotype by low expression of a neuronal-specific enolase p25 transgene.
MA5-12808 was used in western blot to study the partial rescue of the Cdk5 adaptor protein p35 -/- phenotype by overexpression of a neronal-specific enolase p25 transgene
|Patzke H,Maddineni U,Ayala R,Morabito M,Volker J,Dikkes P,Ahlijanian MK,Tsai LH||The Journal of neuroscience : the official journal of the Society for Neuroscience (23:2769)||2003|
Rapid detection of protein aggregates in the brains of Alzheimer patients and transgenic mouse models of amyloidosis.
MA5-12808 was used in western blot to develop a novel method for the rapid detection of beta-amyloid aggregates in Alzheimer's patients and transgenic mouse models
|Xu G,Gonzales V,Borchelt DR||Alzheimer disease and associated disorders (16:191)||2002|
Reduced protein phosphatase 2A activity induces hyperphosphorylation and altered compartmentalization of tau in transgenic mice.
MA5-12808 was used in western blot to study the role of protein phosphatase 2A activity in the hyperphosphorylation and compartmentalization of tau in transgenic mice
|Kins S,Crameri A,Evans DR,Hemmings BA,Nitsch RM,Gotz J||The Journal of biological chemistry (276:38193)||2001|
Hydrogen peroxide induces transient dephosphorylation of tau protein in cultured rat oligodendrocytes.
MA5-12808 was used in western blot to study the transient dephosphorylation of tau induced by hydrogen peroxide in rat oligodendrocytes
|LoPresti P,Konat GW||Neuroscience letters (311:142)||2001|
Generation and characterization of a rabbit monoclonal antibody site-specific for tau O-GlcNAcylated at serine 400.
MA5-12808 was used in immunoprecipitation and western blot to study a site-specific rabbit monoclonal antibody that recognozes serine 400 O-GlcNAcylated tau
|Cameron A,Giacomozzi B,Joyce J,Gray A,Graham D,Ousson S,Neny M,Beher D,Carlson G,O'Moore J,Shearman M,Hering H||FEBS letters (587:3722)||2013|
Abnormal group I metabotropic glutamate receptor expression and signaling in the frontal cortex in Pick disease.
MA5-12808 was used in immunoprecipitation to study abnormal mGluR1 expression and signaling in the frontal cortex in Pick disease
|Dalfó E,Albasanz JL,Rodríguez A,Martín M,Ferrer I||Journal of neuropathology and experimental neurology (64:638)||2005|
Expression of the hyperphosphorylated tau attenuates ER stress-induced apoptosis with upregulation of unfolded protein response.
MA5-12808 was used in immunocytochemistry and western blot to study the role of an upregulation of the unfolded protein response in the mechanism by which tau hyperphosphorylation protects against apoptosis induced by ER stress
|Liu XA,Song J,Jiang Q,Wang Q,Tian Q,Wang JZ||Apoptosis : an international journal on programmed cell death (17:1039)||2012|
A comparative study of Lrrk2 function in primary neuronal cultures.
MA5-12808 was used in immunocytochemistry to investigate the role of Lrrk2 in dendritic neuronal arborization
|Dächsel JC,Behrouz B,Yue M,Beevers JE,Melrose HL,Farrer MJ||Parkinsonism & related disorders (16:650)||2010|
The protein phosphatase PP2A/Bα binds to the microtubule-associated proteins Tau and MAP2 at a motif also recognized by the kinase Fyn: implications for tauopathies.
MA5-12808 was used in western blot to investigate interactions between MAP2 and PP2A/Bα in the brain.
|Sontag JM,Nunbhakdi-Craig V,White CL,Halpain S,Sontag E||The Journal of biological chemistry (287:14984)||2012|
G protein beta1/gamma2 subunit-interacting factor 1; isoform 4; microtubule associated protein tau; microtubule-associated protein tau; microtubule-associated protein tau, isoform 4; neurofibrillary tangle protein; paired helical filament-tau; PHF-tau; protein phosphatase 1, regulatory subunit 103; Tau microtubule-associated protein
AI413597; AW045860; BOS_19251; DDPAC; FTDP-17; MAPT; MAPTL; MSTD; Mtapt; MTBT1; MTBT2; PPND; PPP1R103; pTau; RNPTAU; TAU