Suggested positive control: human testis protein, NIH-3T3 or RAW.
NF-kB (nuclearfactor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing it's translocation to the nucleus (1). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-5). IKK1/a and IKK2/b are phosphorylated by NF-kB-inducing kinase (NIK) (6) and MAP kinase kinase kinase-1 (MEKK1) (7), respectively. Recently, a new IKK family member, TBK1 [(TANK-binding kinase), also known as NAK and T2K] has been identified (8). TANK (TRAF-associated NF-kB activator) interacts with TBK1 and activates NF-kB in a kinase dependent manner.
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Protein Aliases: NAK; NF-kappa-B-activating kinase; NF-kB-activating kinase; serine/threonine protein kinase TBK1; Serine/threonine-protein kinase TBK1; T2K; TANK-binding kinase 1
Gene Aliases: 1200008B05Rik; AI462036; AW048562; BOS_5439; FTDALS4; NAK; T2K; TBK1