Invitrogen

TBK1 Recombinant Rabbit Monoclonal Antibody (4G6C7)

Product Image Gallery

TBK1 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemical analysis of TBK1 in C6 cells using a TBK1 Recombinant Monoclonal antibody (Product # MA5-42812). Blue: DAPI for nuclear staining.

TBK1 Antibody in Western Blot (WB) — Western Blot analysis of TBK1 in various cell lines using a TBK1 Recombinant Monoclonal antibody (Product # MA5-42812) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 30s.

Product Details

MA5-42812

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500 - 1:2,000	-
Immunocytochemistry (ICC/IF)	1:50 - 1:200	-
Immunoprecipitation (IP)	1:50 - 1:200	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Recombinant Monoclonal
Type	Antibody
Clone	4G6C7
Immunogen	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TBK1/NAK (Q9UHD2). View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Positive test controls include: Mouse lung, Mouse testis, Rat testis, 293T, HepG2. The target is usually found in the following locations: Cytoplasm.

Target Information

Nuclear factor kappa B (NF-KB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-KB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-KB is associated with IKB proteins in the cell cytoplasm, which inhibit NF-KB activity. Phosphorylation of I-KB by IKB kinase (IKK) complex leads to degradation of I-KB and activation of NF-KB. The IKK complex contains IKKa, IKKß, and IKKK. A novel IKK related kinase was recently identified and designated TBK1 (TANK-binding kinase 1), NAK (NF-KB-activating kinase), and T2K. NAK/TBK1 activates IKKß through direct phosphorylation. NAK/TBK1 is activated by growth factors and PMA and mediates IKK and NF-kB activation in response to growth factors. NAK/TBK1 functions upstream of NIK and the IKK complex. NAK/TBK1 is also critical in protecting embryonic liver from apoptosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: FLJ11330; NF-kappa-B-activating kinase; NF-kB-activating kinase; serine/threonine protein kinase TBK1; Serine/threonine-protein kinase TBK1; T2K; TANK-binding kinase 1; tbk

Gene Aliases: 1200008B05Rik; AI462036; AW048562; FTDALS4; NAK; T2K; TBK1

UniProt ID: (Human) Q9UHD2, (Mouse) Q9WUN2

Entrez Gene ID: (Human) 29110, (Mouse) 56480, (Rat) 299827

Molecular Function: non-receptor serine/threonine protein kinase protein modifying enzyme