Invitrogen

TBP Monoclonal Antibody (1TBP18)

Product Image Gallery

TBP Antibody — Figure 1--figure supplement 1. Knockdown of ARID1B is synthetically lethal with ARID1A deletion in HCT116 cells. ( A ) Representative Western blot of ARID1A expression in WT and ARID1A -/- HCT116 cells with shRNAs to scrambled control (scr) or ARID1B (KD1, KD2). TBP used as loading control. Three independent experiments were performed. ( B ) Representative western blot of ARID1B expression in WT and ARID1A -/- HCT116 cells with shRNAs to scrambled control (scr) or ARID1B (KD1, KD2). Relative level indicates ARID1B expression normalized to TBP loading control. Average relative level from three independent experiments is shown in Figure 1C . ( C ) CellTiterGlo measurements after 3, 6, or 9 days of growth of WT or ARID1A -/- HCT116 cells expressing shRNAs to scrambled control (scr) or ARID1B (shARID1B KD1, shARID1B KD2) normalized to WT scr control at day 3. Averages shown from two independent biological replicates with standard deviation. ( D ) Western blot of WT and ARID1A -/- HCT116 nuclear extracts separated by glycerol gradient centrifugation. Antibodies used for blotting are indicated. Representative of three biological replicates.

TBP Antibody in Western Blot (WB) — Western blot analysis of TATA binding protein in A) HeLa cell nuclear extract and B) fragments of TATA Binding Protein. Samples were probed with a TATA binding protein monoclonal antibody (Product # MA1-21516) at a dilution of 2 µg/mL.

Product Details

Tested Applications

Dilution

Gel Shift (GS)

Assay dependent

Immunoprecipitation (IP)

Assay dependent

Western Blot (WB)

Assay dependent

Published Applications

Western Blot (WB)

See 4 publications below

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host / Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1TBP18

Immunogen

Synthetic peptide corresponding to the N-terminal residues 1-20 of human, mouse, and rat TBP.

Conjugate

Unconjugated

Form

Liquid

Concentration

1.7 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2199928

Product Specific Information

MA1-21516 detects TATA binding protein (TBP) in human, mouse, and rat samples. This antibody does not cross-react with Drosophila melanogaster, S. cerevisiae, silk worm, or Xenopus laevis.

MA1-21516 has been successfully used in gel shift, immunoprecipitation, and Western blot procedures.

The MA1-21516 immunogen is a synthetic peptide corresponding to the N-terminal residues 1-20 of human, mouse, and rat TBP.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.

Target Information

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. A distinctive feature of TBP is a long string of glutamines in the N-terminal. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. Mutations that expand the number of CAG repeats encoding this polyglutamine tract, and thus increase the length of the polyglutamine string, are associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: TATA box binding protein; TATA sequence-binding protein; TATA-binding factor; TATA-binding protein; TATA-box binding protein N-terminal domain; TATA-box factor; TATA-box-binding protein; TFIID core protein; Transcription initiation factor TFIID TBP subunit

Gene Aliases: GTF2D; GTF2D1; HDL4; SCA17; TBP; TF2D; TFIID

UniProt ID: (Human) P20226, (Mouse) P29037

Entrez Gene ID: (Human) 6908, (Mouse) 21374, (Rat) 117526

Molecular Function: nucleic acid binding transcription factor