Invitrogen

TBP Monoclonal Antibody (1TBP18)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. View Details

6 Published Figures

4 References

View all (19) TBP antibodies

Datasheet

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Datasheet

Questions & Answers

Product Image Gallery

TBP Antibody in Immunocytochemistry (ICC) — Immunofluorescence analysis of TBP was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with TBP Monoclonal Antibody (1TBP18) (Product # MA1-21516) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300 dilution). Panel d represents the merged image showing predominant nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

TBP Antibody in Western Blot (WB) — Western blot was performed using Anti-TBP Monoclonal Antibody (1TBP18) (Product # MA1-21516) and a 41kDa band corresponding to TBP was observed across all cell lines tested, except NIH/3T3 and mouse and rat testes tissues where a 37kDa band was observed. A ~25kDa band (*) corresponding to IgG was observed in mouse testis sample. Nuclear enriched extracts (30 µg lysate) of K-562 (Lane 1), HeLa (Lane 2), A-431 (Lane 3), HT-29 (Lane 4), NIH/3T3 (Lane 5), HEL 92.1.7 (Lane 6), Mouse Testis (Lane 7), Rat Testis (Lane 8 were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (2 µg/mL concentration) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).

TBP Antibody in Knockdown — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with TBP siRNA and decrease in signal intensity was observed in Western Blot application using Anti-TBP Monoclonal Antibody (1TBP18) (Product # MA1-21516). {KD}

TBP Antibody — Figure 5--figure supplement 4. MET protein expression is ARID1A/1B-dependent in HCT116 cells, but can be reconstituted by forced expression of MET. ( A ) Western blot of MET expression in WT and ARID1A-/- cells expressing scrambled or ARID1B shRNA. ( B ) MET protein expression determined by western blotting in WT and ARID1A-/- cells expressing scrambled or ARID1B shRNA. Average relative expression from three independent experiments. ( C ) Western blot of MET and V5 expression in WT and ARID1A-/- cells expressing scrambled or ARID1B shRNA and pLX_Empty or pLX_MET-V5. Representative of three independent experiments from three separate infections. ( D ) MET protein expression determined by western blotting in WT and ARID1A-/- cells expressing scrambled or ARID1B shRNA and pLX_Empty or pLX_MET-V5. Average relative expression from three independent experiments from three separate infections.

Product Details

Applications

Tested Dilution

Publications

Gel Shift (GS)

Assay dependent

Immunocytochemistry (ICC)

1:100

Immunofluorescence (IF)

1:100

Immunoprecipitation (IP)

Assay dependent

Western Blot (WB)

2 µg/mL

View 4 publications 4 publications

Product Specifications

Species

Human, Mouse, Rat

Published species

Human

Expression System

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1TBP18

Immunogen

Synthetic peptide corresponding to the N-terminal residues 1-20 of human, mouse, and rat TBP.

Conjugate

Unconjugated

Form

Liquid

Concentration

0.77 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2199928

Product Specific Information

This antibody does not cross-react with Drosophila melanogaster, S. cerevisiae, silk worm, or Xenopus laevis.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

RICH1 is a GTPase-activating protein (GAP). GAPs stimulate the intrinsic GTP hydrolysis of small G proteins, such as RHOA, RAC1, and CDC42.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: MGC117320; MGC126054; MGC126055; RP1-191N21.3; TATA box binding protein; TATA sequence-binding protein; TATA-binding factor; TATA-binding protein; TATA-box binding protein N-terminal domain; TATA-box factor; tata-box-binding; TATA-box-binding protein; TFIID core protein; Transcription initiation factor TFIID TBP subunit

Gene Aliases: GTF2D; GTF2D1; HDL4; SCA17; TBP; TF2D; TFIID

UniProt ID: (Human) P20226, (Mouse) P29037

Entrez Gene ID: (Human) 6908, (Mouse) 21374, (Rat) 117526

Molecular Function: nucleic acid binding transcription factor