Invitrogen

TBP Monoclonal Antibody (1TBP18)

TBP Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 13

TBP Antibody (MA1-21516) in ICC/IF

Immunofluorescence analysis of TBP was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with TBP Monoclonal Antibody (1TBP18) (Product # MA1-21516) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # ... View More

Product Image Gallery

TBP Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with TBP siRNA and decrease in signal intensity was observed in Western Blot application using Anti-TBP Monoclonal Antibody (1TBP18) (Product # MA1-21516). {KD}

Product Details

MA1-21516

Applications

Tested Dilution

Publications

Western Blot (WB)

1:300-1:500

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

1:100

Immunoprecipitation (IP)

Assay-dependent

Gel Shift (GS)

Assay-dependent

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1TBP18

Immunogen

Recombinant human TBP.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.70 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2199928

Product Specific Information

This antibody does not cross-react with Drosophila melanogaster, S. cerevisiae, silk worm, or Xenopus laevis.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

RICH1 is a GTPase-activating protein (GAP). GAPs stimulate the intrinsic GTP hydrolysis of small G proteins, such as RHOA, RAC1, and CDC42.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Chromatin accessibility underlies synthetic lethality of SWI/SNF subunits in ARID1A-mutant cancers.

eLife

Kelso TWR,Porter DK,Amaral ML,Shokhirev MN,Benner C,Hargreaves DC

Published figure using TBP monoclonal antibody (Product # MA1-21516) in Western Blot

Mon Oct 02 00:00:00 UTC 2017

Loss of Runx2 sensitises osteosarcoma to chemotherapy-induced apoptosis.

British journal of cancer

Roos A,Satterfield L,Zhao S,Fuja D,Shuck R,Hicks MJ,Donehower LA,Yustein JT

MA1-21516 was used in western blot to determine the sensitization of osteosarcoma to chemotherapy-induced apoptosis by a loss of Runx2

Tue Nov 03 00:00:00 UTC 2015

The RIP140 gene is a transcriptional target of E2F1.

PloS one

Docquier A,Augereau P,Lapierre M,Harmand PO,Badia E,Annicotte JS,Fajas L,Cavaillès V

Published figure using TBP monoclonal antibody (Product # MA1-21516) in Western Blot

Mon Dec 17 00:00:00 UTC 2012

Wild-type p53-induced phosphatase 1 dephosphorylates histone variant gamma-H2AX and suppresses DNA double strand break repair.

The Journal of biological chemistry

Moon SH,Lin L,Zhang X,Nguyen TA,Darlington Y,Waldman AS,Lu X,Donehower LA

Published figure using TBP monoclonal antibody (Product # MA1-21516) in Western Blot

Fri Apr 23 00:00:00 UTC 2010

Bioinformatics

Protein Aliases: MGC117320; MGC126054; MGC126055; RP1-191N21.3; TATA box binding protein; TATA sequence-binding protein; TATA-binding factor; TATA-binding protein; TATA-box binding protein N-terminal domain; TATA-box factor; tata-box-binding; TATA-box-binding protein; TFIID core protein; Transcription initiation factor TFIID TBP subunit

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Gene Aliases: GTF2D; GTF2D1; HDL4; SCA17; TBP; TF2D; TFIID

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UniProt ID: (Human) P20226, (Mouse) P29037

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Entrez Gene ID: (Human) 6908, (Mouse) 21374, (Rat) 117526

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding RNA polymerase II repressing transcription factor binding transcription factor activity, sequence-specific DNA binding protein binding transcription factor binding enzyme binding transcription regulatory region DNA binding repressing transcription factor binding DNA binding general transcription factor

Process(es)

transcription initiation from RNA polymerase I promoter transcription elongation from RNA polymerase I promoter termination of RNA polymerase I transcription transcription from RNA polymerase II promoter transcription initiation from RNA polymerase II promoter transcription elongation from RNA polymerase II promoter transcription from RNA polymerase III promoter spermatogenesis viral process snRNA transcription from RNA polymerase II promoter positive regulation of gene expression, epigenetic positive regulation of transcription, DNA-templated regulation of signal transduction by p53 class mediator transcription, DNA-templated DNA-templated transcription, initiation regulation of transcription, DNA-templated

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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TBP Monoclonal Antibody (1TBP18)

TBP Antibody (MA1-21516) in ICC/IF

Product Details

MA1-21516

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Gel Shift (GS)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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