Invitrogen

TBP Monoclonal Antibody (1TBP18)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

6 Published Figures

4 References

View all (26) TBP antibodies

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Protocols

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Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

TBP Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of TBP was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with TBP Monoclonal Antibody (1TBP18) (Product # MA1-21516) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300 dilution). Panel d represents the merged image showing predominant nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

TBP Antibody in Western Blot (WB) — Western blot was performed using Anti-TBP Monoclonal Antibody (1TBP18) (Product # MA1-21516) and a 41kDa band corresponding to TBP was observed across all cell lines tested, except NIH/3T3 and mouse and rat testes tissues where a 37kDa band was observed. A ~25kDa band (*) corresponding to IgG was observed in mouse testis sample. Nuclear enriched extracts (30 µg lysate) of K-562 (Lane 1), HeLa (Lane 2), A-431 (Lane 3), HT-29 (Lane 4), NIH/3T3 (Lane 5), HEL 92.1.7 (Lane 6), Mouse Testis (Lane 7), Rat Testis (Lane 8 were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (2 µg/mL concentration) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).

TBP Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with TBP siRNA and decrease in signal intensity was observed in Western Blot application using Anti-TBP Monoclonal Antibody (1TBP18) (Product # MA1-21516). {KD}

Product Details

MA1-21516

Applications	Tested Dilution	Publications
Western Blot (WB)	2 µg/mL	View 4 publications 4 publications
Immunocytochemistry (ICC/IF)	1:100	-
Immunoprecipitation (IP)	Assay-dependent	-
Gel Shift (GS)	Assay-dependent	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	1TBP18
Immunogen	Synthetic peptide corresponding to the N-terminal residues 1-20 of human TBP. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.70 mg/mL
Purification	Protein G
Storage buffer	PBS, pH 7.4
Contains	no preservative
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2199928

Product Specific Information

This antibody does not cross-react with Drosophila melanogaster, S. cerevisiae, silk worm, or Xenopus laevis.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

RICH1 is a GTPase-activating protein (GAP). GAPs stimulate the intrinsic GTP hydrolysis of small G proteins, such as RHOA, RAC1, and CDC42.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Chromatin accessibility underlies synthetic lethality of SWI/SNF subunits in ARID1A-mutant cancers.

eLife

Kelso TWR,Porter DK,Amaral ML,Shokhirev MN,Benner C,Hargreaves DC

Published figure using TBP monoclonal antibody (Product # MA1-21516) in Western Blot

Mon Oct 02 00:00:00 EDT 2017

Loss of Runx2 sensitises osteosarcoma to chemotherapy-induced apoptosis.

British journal of cancer

Roos A,Satterfield L,Zhao S,Fuja D,Shuck R,Hicks MJ,Donehower LA,Yustein JT

MA1-21516 was used in western blot to determine the sensitization of osteosarcoma to chemotherapy-induced apoptosis by a loss of Runx2

Tue Nov 03 00:00:00 EST 2015

The RIP140 gene is a transcriptional target of E2F1.

PloS one

Docquier A,Augereau P,Lapierre M,Harmand PO,Badia E,Annicotte JS,Fajas L,Cavaillès V

MA1-21516 was used in western blot to study the role of the RIP140 gene as a new transcriptional target of E2F1

Mon Dec 17 00:00:00 EST 2012

Wild-type p53-induced phosphatase 1 dephosphorylates histone variant gamma-H2AX and suppresses DNA double strand break repair.

The Journal of biological chemistry

Moon SH,Lin L,Zhang X,Nguyen TA,Darlington Y,Waldman AS,Lu X,Donehower LA

MA1-21516 was used in western blot to investigate the regulation of gamma-H2AX's phosphorylation status by WIP1 and its relationship with the repair of double-stranded DNA damage

Fri Apr 23 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: MGC117320; MGC126054; MGC126055; RP1-191N21.3; TATA box binding protein; TATA sequence-binding protein; TATA-binding factor; TATA-binding protein; TATA-box binding protein N-terminal domain; TATA-box factor; tata-box-binding; TATA-box-binding protein; TFIID core protein; Transcription initiation factor TFIID TBP subunit

Gene Aliases: GTF2D; GTF2D1; HDL4; SCA17; TBP; TF2D; TFIID

UniProt ID: (Human) P20226, (Mouse) P29037

Entrez Gene ID: (Human) 6908, (Mouse) 21374, (Rat) 117526

Molecular Function: general transcription factor