|Tested species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Mouse recombinant protein from C-terminal region Tpit.|
|Purification||Ammonium sulfate precipitation|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:2,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-803 detects endogenous Tpit from mouse AtT-20 cell lysates.
PA1-803 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~48 kDa protein representing Tpit from AtT-20 cells.
PA1-803 immunizing protein, bacterially produced, corresponds to the mouse C-terminal region of Tpit.
Tpit (TBX19, T-Box protein 19) is a highly cell-restricted transcription factor belonging to the T-Box family. Tpit is required for pro-opeiomelanocortin (POMC) gene expression and differentiation of corticotroph and melanotroph cells. Tpit gene mutations have been shown to be directly correlated to pituitary adrenocorticotropin (ACTH) deficiencies. Tpit functions as a transcriptional activator by recruiting the p160 family of coactivators.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.