|Tested species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||His-tagged fusion protein to residues 31-331 of the human TCF.|
|Storage buffer||0.1M tris glycine, pH 7.4, with 30% glycerol, 0.15M NaCl|
|Contains||0.08% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Gel Shift (GS)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
TCF 4 (T cell factor 4) is a member of the TCF/LEF (lymphoid enhancer factor) family of transcription factors. Members of this family along with b catenin, are involved in mediating Wnt signaling by transactivating downstream genes. TCF 4 generates transcriptionally active complexes with b catenin in colon carcinoma cells. Expression of TCF 4 is highest in the CNS and intestinal epithelium where Wnt signaling also plays an essential role in development. Aberrant TCF 4 transcription activity has been implicated in carcinogenesis of intestinal and mammary epithlia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.