|Tested species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||His-tagged fusion protein, corresponding to amino acids 31-331 of Human TCF4|
|Storage buffer||0.07M tris glycine, pH 7.4, with 0.105M NaCl, 30% glycerol|
|Contains||0.035% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||0.5-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-91858 detects TCF4 from human and mouse samples.
MA1-91858 has been successfully used in immunohistochemistry, immunocytochemistry, immunoprecipitation, gel shift, ICC, IF, and Western blot applications. Detects a band at approximately 66 kDa. Positive control of SW620 whole cell lysate suggested.
The MA1-91858 immunogen is the His-tagged fusion protein, corresponding to amino acids 31-331 of Human TCF4
Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze/thaw cycles.
TCF7L2, or TCF4, is a high mobility group (HMG) box-containing transcription factor that plays a key role in the Wnt signaling pathway. TCF7L2 has been implicated in glucose homeostasis through the regulation of pro-glucagon gene expression, which encodes glucagon-like peptide 1 (GLP-1). Genetic variants of TCF7L2 have been linked to type 2 diabetes, and it has specifically been shown to have an important role in the regulation of both beta-cell survival and function. Several splicing isoforms for TCF7L2 have been shown to be differentially expressed in tissues during cancer progression, and gene targeting studies indicate that TCF7L2 is required to maintain the crypt stem cells of the small intestine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.