Flow cytometry analysis of TCR V alpha 2 on TCR V alpha 2 positive MOLT16 cells (left) or negative control Jurkat cells (right panel). Equal numbers of cells were stained with a TCR V alpha 2 (F1) monoclonal antibody (Product # TCR1663) followed by FITC labeled secondary antibody, or FITC labeled secondary antibody alone. 5ul of primary antibody were used per test. All antibody incubations were performed for 30 minutes at room temperature. A representative 10,000 cells were acquired for each sample.
|Tested species reactivity||Human, Non-human primate|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Human TCR V alpha-2|
|Storage buffer||PBS with 0.5% BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1-2 µg/test|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response. TCR recognition of self-peptides has been linked to autoimmune disease. Mutant self-peptides have been associated with tumors. Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome. Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). The autoantibodies block TH1-mediated inflammatory autodestructive reactions and are believed to be a method by which the immune system compensates for disease (ref5). T Cell and TCR Diversity Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP). A small number of T cells lack both CD4 and CD8 (double negative, DN). Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders. Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Altered expression of T cell immunoglobulin-mucin (TIM) molecules in bronchoalveolar lavage CD4+ T cells in sarcoidosis.
TCR1663 was used in flow cytometry to investigate the expression status of T cell immunoglobulin-mucin molecules in sarcoidosis
|Idali F,Wahlström J,Dahlberg B,Khademi M,Olsson T,Eklund A,Grunewald J||Respiratory research (10:null)||2009|
Identification of an enhancer agonist cytotoxic T lymphocyte peptide from human carcinoembryonic antigen.
TCR1663 was used in flow cytometry to develop and optimize a novel vaccination strategy
|Zaremba S,Barzaga E,Zhu M,Soares N,Tsang KY,Schlom J||Cancer research (57:4570)||1997|
NKG2D-mediated antitumor activity by tumor-infiltrating lymphocytes and antigen-specific T-cell clones isolated from melanoma patients.
TCR1663 was used in immunohistochemistry to study the role of NKG2D+ T cells in the immunologic response against tumors
|Maccalli C,Nonaka D,Piris A,Pende D,Rivoltini L,Castelli C,Parmiani G||Clinical cancer research : an official journal of the American Association for Cancer Research (13:7459)||2007|
Evidence for restricted Vbeta usage in the leukemic phase of cutaneous T cell lymphoma.
TCR1663 was used in immunohistochemistry to study the change of Vbeta chain of T cell receptors during the leukemic phase of cutaneous T cell lymphoma
|Vonderheid EC,Boselli CM,Conroy M,Casaus L,Espinoza LC,Venkataramani P,Bigler RD,Hou JS||The Journal of investigative dermatology (124:651)||2005|
Identical alpha-chain T-cell receptor transcripts are present on T cells infiltrating coronary arteries of human cardiac allografts with chronic rejection.
TCR1663 was used in immunohistochemistry to investigate the expression of alpha-chain T-cell receptor in a subset of T cells
|Xu B,Sakkas LI,Goldman BI,Jeevanandam V,Gaughan J,Oleszak EL,Platsoucas CD||Cellular immunology (225:75)||2003|
Hypopigmented mycosis fungoides: frequent expression of a CD8+ T-cell phenotype.
TCR1663 was used in immunohistochemistry to investigate the expression of CD8+ T cells in patients with hypopigmented mycosis fungoides
|El-Shabrawi-Caelen L,Cerroni L,Medeiros LJ,McCalmont TH||The American journal of surgical pathology (26:450)||2002|
Unaltered distribution of laminins, fibronectin, and tenascin in celiac intestinal mucosa.
TCR1663 was used in immunohistochemistry to investigate the expression and distribution of laminins, fibronectin, and tenascin in celiac intestinal mucosa
|Korhonen M,Ormio M,Burgeson RE,Virtanen I,Savilahti E||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (48:1011)||2000|
Small bowel T cells, HLA class II antigen DR, and GroEL stress protein in IgA nephropathy.
TCR1663 was used in immunohistochemistry to study the roles of intestinal mucosa in the pathogenesis of IgA nephropathy
|Rantala I,Collin P,Holm K,Kainulainen H,Mustonen J,Mäki M||Kidney international (55:2274)||1999|
Immunological study on CD3 defective cutaneous T cell lymphoma cells from a patient with Sézary syndrome.
TCR1663 was used in immunohistochemistry to characterize T cell lymphoma cells from a Sezary syndrome patient
|Sano S,Matsui Y,Itami S,Yoshikawa K||Clinical and experimental immunology (113:190)||1998|
Demonstration of clonality in T-cell lymphoma using an anti-T-cell receptor variable region antibody panel.
TCR1663 was used in immunohistochemistry to study the tumorigenesis of T-cell lymphoma
|O'Grady J,Krajewski AS,Ramage EF||Histopathology (17:553)||1990|
Targeting the Wilms tumor antigen 1 by TCR gene transfer: TCR variants improve tetramer binding but not the function of gene modified human T cells.
TCR1663 was used in western blot to study the effect of the Wilms tumor antigen 1 disruption
|Thomas S,Xue SA,Cesco-Gaspere M,San José E,Hart DP,Wong V,Debets R,Alarcon B,Morris E,Stauss HJ||Journal of immunology (Baltimore, Md. : 1950) (179:5803)||2007|