Staining of C57Bl/6 splenocytes with Anti-Mouse CD3e APC (Product # 17-0031) and 0.125 µg of Rat IgG2a K Isotype Control PE (Product # 12-4321) (left) or 0.125 µg of Anti-Mouse V beta 10b TCR PE (right). Cells in the lymphocyte gate were used for analysis.
|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rat / IgG2a, lambda|
|Storage buffer||PBS, pH 7.2, with 0.1% gelatin|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, store in dark, DO NOT FREEZE!|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.25 µg/test|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Description: The B21.5 monoclonal antibody reacts with the Vbeta10 T Cell Receptor (TCR) in mice of the b haplotype of the TCR Vbeta gene complex, which includes AKR, BALB/c, CBA, C3H/He, C57BL, DBA/1 and DBA/2 mice. It does not react with Vbeta10 in mice of the a or c haplotypes of the TCR Vbeta gene complex, including C57BR, C57L, SJL, and SWR mice.
Applications Reported: This B21.5 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This B21.5 antibody has been tested by flow cytometric analysis of C57Bl/6 mouse lymph node cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response. TCR recognition of self-peptides has been linked to autoimmune disease. Mutant self-peptides have been associated with tumors. Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome. Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). The autoantibodies block TH1-mediated inflammatory autodestructive reactions and are believed to be a method by which the immune system compensates for disease (ref5). T Cell and TCR Diversity Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP). A small number of T cells lack both CD4 and CD8 (double negative, DN). Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders. Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
||Monoclonal antibodies raised against engineered soluble mouse T cell receptors and specific for V alpha 8-, V beta 2- or V beta 10-bearing T cells.||Necker A,Rebaï N,Matthes M,Jouvin-Marche E,Cazenave PA,Swarnworawong P,Palmer E,MacDonald HR,Malissen B||European journal of immunology (21:3035)||1991|
|Not Applicable||Not Cited||Monoclonal antibodies raised against engineered soluble mouse T cell receptors and specific for V alpha 8-, V beta 2- or V beta 10-bearing T cells.||Necker A,Rebaï N,Matthes M,Jouvin-Marche E,Cazenave PA,Swarnworawong P,Palmer E,MacDonald HR,Malissen B||European journal of immunology (21:3035)||1991|