|Tested species reactivity||Human|
|Published species reactivity||Mouse, Human|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Human TCR V beta 12|
|Storage buffer||PBS with 0.5% BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1-2 µg/test|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|T-Cell Activation (TCA)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
TCR1654 targets TCR V beta 12 in FACS, IHC(F), IP, and TCA applications and shows reactivity with Human samples.
The TCR1654 immunogen is human TCR V beta 12.
The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response. TCR recognition of self-peptides has been linked to autoimmune disease. Mutant self-peptides have been associated with tumors. Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome. Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). The autoantibodies block TH1-mediated inflammatory autodestructive reactions and are believed to be a method by which the immune system compensates for disease (ref5). T Cell and TCR Diversity Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP). A small number of T cells lack both CD4 and CD8 (double negative, DN). Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders. Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Mutant HSP70 reverses autoimmune depigmentation in vitiligo.
TCR1654 was used in immunohistochemistry to study the ability of a HSP70Q435A mutant to reverse vitiligo autoimmune depigmentation
|Mosenson JA,Zloza A,Nieland JD,Garrett-Mayer E,Eby JM,Huelsmann EJ,Kumar P,Denman CJ,Lacek AT,Kohlhapp FJ,Alamiri A,Hughes T,Bines SD,Kaufman HL,Overbeck A,Mehrotra S,Hernandez C,Nishimura MI,Guevara-Patino JA,Le Poole IC||Science translational medicine (5:null)||2013|
A coreceptor-independent transgenic human TCR mediates anti-tumor and anti-self immunity in mice.
TCR1654 was used in immunohistochemistry to study the role of a coreceptor-independent transgenic human TCR in anti-tumor and anti-self immunity in mice
|Mehrotra S,Al-Khami AA,Klarquist J,Husain S,Naga O,Eby JM,Murali AK,Lyons GE,Li M,Spivey ND,Norell H,Martins da Palma T,Onicescu G,Diaz-Montero CM,Garrett-Mayer E,Cole DJ,Le Poole IC,Nishimura MI||Journal of immunology (Baltimore, Md. : 1950) (189:1627)||2012|
Evidence for restricted Vbeta usage in the leukemic phase of cutaneous T cell lymphoma.
TCR1654 was used in immunohistochemistry to investigate the status of Vbeta usage in cutaneous T cell lymphoma
|Vonderheid EC,Boselli CM,Conroy M,Casaus L,Espinoza LC,Venkataramani P,Bigler RD,Hou JS||The Journal of investigative dermatology (124:651)||2005|
Immunological study on CD3 defective cutaneous T cell lymphoma cells from a patient with Sézary syndrome.
TCR1654 was used in immunohistochemistry to study the effect of CD3 surface expression on cutaneous T cell lymphoma cells
|Sano S,Matsui Y,Itami S,Yoshikawa K||Clinical and experimental immunology (113:190)||1998|
A rapid crosstalk of human gammadelta T cells and monocytes drives the acute inflammation in bacterial infections.
TCR1654 was used in flow cytometry to investigate the importance of the crosstalk between V gamma 9/V delta 2 T cells and monocytes in immunological response to microbial infection
|Eberl M,Roberts GW,Meuter S,Williams JD,Topley N,Moser B||PLoS pathogens (5:null)||2009|
Selective activation of TCR-gammadelta+ cells in endemic Burkitt's lymphoma.
TCR1654 was used in flow cytometry to investigate different T-cell compositions and activation status in Plasmodium falciparum-exposed Ghanaian children with and without endemic Burkitt lymphoma
|Futagbi G,Welbeck JE,Tetteh JK,Hviid L,Akanmori BD||Malaria journal (6:null)||2007|
Recognition of nonpeptide antigens by human V gamma 9V delta 2 T cells requires contact with cells of human origin.
TCR1654 was used in flow cytometry to study the mechanism for nonpeptide antigen recognition by human Vgamma9Vdelta2 T cells
|Green AE,Lissina A,Hutchinson SL,Hewitt RE,Temple B,James D,Boulter JM,Price DA,Sewell AK||Clinical and experimental immunology (136:472)||2004|
Effects of HIV-1 peptides on T-cell receptor variable beta chain families.
TCR1654 was used in flow cytometry to study the role of HIV-1 peptides in TCR Vbeta families
|Eick A,Larned J,Jason J||Human immunology (61:993)||2000|
Transient T cell receptor beta-chain variable region-specific expansions of CD4+ and CD8+ T cells during the early phase of pediatric human immunodeficiency virus infection: characterization of expanded cell populations by T cell receptor phenotyping.
TCR1654 was used in flow cytometry to study the expanded CD4+ and CD8+ T cell populations by TCR phenotyping
|Soudeyns H,Champagne P,Holloway CL,Silvestri GU,Ringuette N,Samson J,Lapointe N,Sékaly RP||The Journal of infectious diseases (181:107)||2000|
Structural dichotomy of staphylococcal enterotoxin C superantigens leading to MHC class II-independent activation of T lymphocytes.
TCR1654 was used in flow cytometry to study the effect of staphylococcal enterotoxin C superantigens on T lymphocyte activation
|Lamphear JG,Bohach GA,Rich RR||Journal of immunology (Baltimore, Md. : 1950) (160:2107)||1998|
Identification of an enhancer agonist cytotoxic T lymphocyte peptide from human carcinoembryonic antigen.
TCR1654 was used in flow cytometry to identify a specific peptide from human carcinoembryonic antigen to enhance the immunogenicity of tumor cell self-antigens
|Zaremba S,Barzaga E,Zhu M,Soares N,Tsang KY,Schlom J||Cancer research (57:4570)||1997|
The bisphosphonate acute phase response: rapid and copious production of proinflammatory cytokines by peripheral blood gd T cells in response to aminobisphosphonates is inhibited by statins.
TCR1654 was used in immunocytochemistry to characterize the acute phase response by bisphosphonates
|Hewitt RE,Lissina A,Green AE,Slay ES,Price DA,Sewell AK||Clinical and experimental immunology (139:101)||2005|
Large-scale early in vitro response to actinobacillus actinomycetemcomitans suggests superantigenic activation of T-cells.
TCR1654 was used in immunocytochemistry to study the mechanism for T cell response to Actinobacillus actinomycetemcomitans
|Zadeh HH,Nalbant A,Park K||Journal of dental research (80:356)||2001|