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Flow cytometry analysis of TCR V beta 12 showing staining in PBMC cells (green) compared to an isotype control (blue). Human blood was collected, combined with a hydrophilic polysaccharide, centrifuged, transferred to a conical tube and washed with PBS. 50 ul of cell solution was added to each tube at a dilution of 2x10^7 cells/ml, followed by the addition of 50 ul of isotype control and primary antibody (Product # TCR2654) at a dilution of 10ul/test. Cells were incubated for 30 min at 4°C and washed with a cell buffer, followed by incubation with a DyLight 488-conjugated goat anti-mouse IgG (H+L) secondary for 30 min at 4°C in the dark. FACS analysis was performed using 400 ul of cell buffer.
|Tested species reactivity||Human|
|Published species reactivity||Mouse, Human|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Human TCR V beta 12|
|Storage buffer||PBS with 0.5% BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||10 ul/test|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|T-Cell Activation (TCA)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
TCR2654 targets TCR V beta 12 in FACS, IHC(F), IP, and TCA applications and shows reactivity with Human samples.
The TCR2654 immunogen is human TCR V beta 12.
The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response. TCR recognition of self-peptides has been linked to autoimmune disease. Mutant self-peptides have been associated with tumors. Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome. Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). The autoantibodies block TH1-mediated inflammatory autodestructive reactions and are believed to be a method by which the immune system compensates for disease (ref5). T Cell and TCR Diversity Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP). A small number of T cells lack both CD4 and CD8 (double negative, DN). Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders. Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
A coreceptor-independent transgenic human TCR mediates anti-tumor and anti-self immunity in mice.
TCR2654 was used in immunohistochemistry to study the role of a coreceptor-independent transgenic human TCR in anti-tumor and anti-self immunity in mice
|Mehrotra S,Al-Khami AA,Klarquist J,Husain S,Naga O,Eby JM,Murali AK,Lyons GE,Li M,Spivey ND,Norell H,Martins da Palma T,Onicescu G,Diaz-Montero CM,Garrett-Mayer E,Cole DJ,Le Poole IC,Nishimura MI||Journal of immunology (Baltimore, Md. : 1950) (189:1627)||2012|
Depletion of Vbeta5.2/5.3 T cells with a humanized antibody in patients with multiple sclerosis.
TCR2654 was used in flow cytometry to study the selective depletion of Vbeta5.2/5.3 T cells in multiple sclerosis patients
|Olsson T,Edenius C,Ferm M,Samuelson P,Torrång A,Wallström E,Khademi M,Andersson M,Arfors L||European journal of neurology (9:153)||2002|