Flow cytometry analysis of TCR V beta 3.1 in PBMC cells compared to an isotype control (blue). Human blood was collected, combined with a hydrophilic polysaccharide, centrifuged, transferred to a conical tube and washed with PBS. 50 ul of cell solution was added to each tube at a dilution of 2x10^7 cells/ml, followed by the addition of 50 ul of isotype control and primary antibody (Product # TCR2740) at a dilution of 1:20. Cells were incubated for 30 min at 4°C and washed with a cell buffer, followed by incubation with a DyLight 488-conjugated goat anti-mouse IgG (H+L) secondary for 30 min at 4°C in the dark. FACS analysis was performed using 400 ul of cell buffer.
|Tested species reactivity||Human|
|Published species reactivity||Non-human primate, Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human TCR V beta 3.1|
|Storage buffer||PBS with 0.5% BSA, glycerol|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
TCR2740 targets TCR V beta 3.1 in FACS applications and shows reactivity with Human samples.
The TCR2740 immunogen is human TCR Vß3.1.
The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response. TCR recognition of self-peptides has been linked to autoimmune disease. Mutant self-peptides have been associated with tumors. Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome. Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). The autoantibodies block TH1-mediated inflammatory autodestructive reactions and are believed to be a method by which the immune system compensates for disease (ref5). T Cell and TCR Diversity Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP). A small number of T cells lack both CD4 and CD8 (double negative, DN). Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders. Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Non-human primate||Not Cited||
Phosphoantigen-activated V gamma 2V delta 2 T cells antagonize IL-2-induced CD4+CD25+Foxp3+ T regulatory cells in mycobacterial infection.
TCR2740 was used in flow cytometry to investigate the effect of phosphoantigen-activated V gamma 2V delta 2 T cells for interleukin-2-induced CD4+CD25+Foxp3+ T regulatory cells in mycobacterial infection
|Gong G,Shao L,Wang Y,Chen CY,Huang D,Yao S,Zhan X,Sicard H,Wang R,Chen ZW||Blood (113:837)||2009|
Depletion of Vbeta5.2/5.3 T cells with a humanized antibody in patients with multiple sclerosis.
TCR2740 was used in flow cytometry to study the selective depletion of Vbeta5.2/5.3 T cells in MS patients
|Olsson T,Edenius C,Ferm M,Samuelson P,Torrång A,Wallström E,Khademi M,Andersson M,Arfors L||European journal of neurology (9:153)||2002|
Effects of HIV-1 peptides on T-cell receptor variable beta chain families.
TCR2740 was used in flow cytometry to study the role of HIV-1 peptides in the expansion and deletion of TCR Vbeta families
|Eick A,Larned J,Jason J||Human immunology (61:993)||2000|
Alpha beta T cell response to Toxoplasma gondii in previously unexposed individuals.
TCR2740 was used in flow cytometry to characterize the alpha beta T cell immune response to Toxoplasma gondii
|Subauste CS,Fuh F,de Waal Malefyt R,Remington JS||Journal of immunology (Baltimore, Md. : 1950) (160:3403)||1998|
Structural dichotomy of staphylococcal enterotoxin C superantigens leading to MHC class II-independent activation of T lymphocytes.
TCR2740 was used in flow cytometry to study the effect of staphylococcal enterotoxin C superantigens on T lymphocyte activation
|Lamphear JG,Bohach GA,Rich RR||Journal of immunology (Baltimore, Md. : 1950) (160:2107)||1998|
Identification of an enhancer agonist cytotoxic T lymphocyte peptide from human carcinoembryonic antigen.
TCR2740 was used in flow cytometry to develop and optimize a novel vaccination strategy
|Zaremba S,Barzaga E,Zhu M,Soares N,Tsang KY,Schlom J||Cancer research (57:4570)||1997|
Oligoclonal expansions of CD4+ and CD8+ T-cells in the target organ of patients with biliary atresia.
TCR2740 was used in immunocytochemistry to characterize CD4+ and CD8+ T-cell expansion in biliary atresia
|Mack CL,Falta MT,Sullivan AK,Karrer F,Sokol RJ,Freed BM,Fontenot AP||Gastroenterology (133:278)||2007|
Restricted T cell receptor BV gene usage in the lungs and muscles of patients with idiopathic inflammatory myopathies.
TCR2740 was used in immunohistochemistry to investigate the T cell receptor BV gene expression in various tissues in patients with idiopathic inflammatory myopathies
|Englund P,Wahlström J,Fathi M,Rasmussen E,Grunewald J,Tornling G,Lundberg IE||Arthritis and rheumatism (56:372)||2007|
Selective accumulation of related CD4+ T cell clones in the synovial fluid of patients with rheumatoid arthritis.
TCR2740 was used in immunohistochemistry to characterize the role of CD4+ T cells in rheumatoid arthritis
|Striebich CC,Falta MT,Wang Y,Bill J,Kotzin BL||Journal of immunology (Baltimore, Md. : 1950) (161:4428)||1998|