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Flow cytometry analysis of TCR V beta 5a in PBMC cells (green) compared to an isotype control (blue). Human blood was collected, combined with a hydrophilic polysaccharide, centrifuged, transferred to a conical tube and washed with PBS. 50 ul of cell solution was added to each tube at a dilution of 2x10^7 cells/ml, followed by the addition of 50 ul of isotype control and primary antibody (Product # TCR2642) at a dilution of 2 ug/test. Cells were incubated for 30 min at 4°C and washed with a cell buffer, followed by incubation with a DyLight 488-conjugated goat anti-mouse IgG (H+L) secondary for 30 min at 4°C in the dark. FACS analysis was performed using 400 ul of cell buffer.
|Tested species reactivity||Human, Mouse, Primate|
|Published species reactivity||Primate, Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human TCR V beta 5(a)|
|Storage buffer||PBS with glycerol, 0.5% BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||2 ug/test|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|T-Cell Activation (TCA)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Flow Cytometry (Flow)||See 5 publications below|
TCR2642 targets TCR V beta 5a in FACS, IHC(F), and TCA applications and shows reactivity with Human, Mouse and Non-human primate samples.
The TCR2642 immunogen is human TCR V beta 5(a).
The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response. TCR recognition of self-peptides has been linked to autoimmune disease. Mutant self-peptides have been associated with tumors. Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome. Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). The autoantibodies block TH1-mediated inflammatory autodestructive reactions and are believed to be a method by which the immune system compensates for disease (ref5). T Cell and TCR Diversity Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP). A small number of T cells lack both CD4 and CD8 (double negative, DN). Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders. Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Single-chain VαVβ T-cell receptors function without mispairing with endogenous TCR chains.
TCR2642 was used in flow cytometry to study the ability of engineered single-chain V?V? T-cell receptors to function without mispairing with endogenous TCR chains
|Aggen DH,Chervin AS,Schmitt TM,Engels B,Stone JD,Richman SA,Piepenbrink KH,Baker BM,Greenberg PD,Schreiber H,Kranz DM||Gene therapy (19:365)||2012|
Phosphoantigen-activated V gamma 2V delta 2 T cells antagonize IL-2-induced CD4+CD25+Foxp3+ T regulatory cells in mycobacterial infection.
TCR2642 was used in flow cytometry to investigate the effect of phosphoantigen-activated V gamma 2V delta 2 T cells for interleukin-2-induced CD4+CD25+Foxp3+ T regulatory cells in mycobacterial infection
|Gong G,Shao L,Wang Y,Chen CY,Huang D,Yao S,Zhan X,Sicard H,Wang R,Chen ZW||Blood (113:837)||2009|
Effects of HIV-1 peptides on T-cell receptor variable beta chain families.
TCR2642 was used in flow cytometry to study the role of HIV-1 peptides in the expansion and deletion of TCR Vbeta families
|Eick A,Larned J,Jason J||Human immunology (61:993)||2000|
The T cell receptor repertoire of CD8+CD28- T lymphocytes is dominated by expanded clones that persist over time.
TCR2642 was used in flow cytometry to study the T cell receptor repertoire in CD8+CD28- T lymphocytes
|Mugnaini EN,Egeland T,Spurkland A,Brinchmann JE||Clinical and experimental immunology (117:298)||1999|
Rapid screening of T-cell receptor (TCR) variable gene usage by multiplex PCR: application for assessment of clonal composition.
TCR2642 was used in flow cytometry to evaluate the multiplex PCR for the screening of T-cell receptor variable gene
|Akatsuka Y,Martin EG,Madonik A,Barsoukov AA,Hansen JA||Tissue antigens (53:122)||1999|