Invitrogen

TCR V beta 5a Monoclonal Antibody (1C1), FITC

Product Image Gallery

TCR V beta 5a Antibody in Flow Cytometry (Flow) — Flow cytometry analysis of TCR V beta 5a in PBMC cells (green) compared to an isotype control (blue). Human blood was collected, combined with a hydrophilic polysaccharide, centrifuged, transferred to a conical tube and washed with PBS. 50 µL of cell solution was added to each tube at a dilution of 2x10^7 cells/mL, followed by the addition of 50 µL of isotype control and primary antibody (Product # TCR2642) at a dilution of 2 µg/test. Cells were incubated for 30 min at 4°C and washed with a cell buffer, followed by incubation with a DyLight 488-conjugated goat anti-mouse IgG (H+L) secondary for 30 min at 4°C in the dark. FACS analysis was performed using 400 µL of cell buffer.

Product Details

TCR2642

Applications	Tested Dilution	Publications
Immunohistochemistry (Frozen) (IHC (F))	Assay-dependent	-
Flow Cytometry (Flow)	2 µg/test	View 5 publications 5 publications
T-Cell Activation (TCA)	Assay-dependent	-

Product Specifications
Species Reactivity	Human, Mouse, Non-human primate
Published species	Human, Non-human primate
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	1C1
Immunogen	Human TCR V beta 5(a)
Conjugate	FITC Unconjugated
Excitation/Emission Max	498/517 nm View spectra
Form	Liquid
Concentration	0.5 mg/mL
Purification	Protein G
Storage buffer	PBS with 0.5% BSA
Contains	0.1% sodium azide
Storage conditions	4° C, store in dark
RRID	AB_223621

Product Specific Information

TCR2642 targets TCR V beta 5a in FACS, IHC(F), and TCA applications and shows reactivity with Human, Mouse and Non-human primate samples.

The TCR2642 immunogen is human TCR V beta 5(a).

Target Information

The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response. TCR recognition of self-peptides has been linked to autoimmune disease. Mutant self-peptides have been associated with tumors. Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome. Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). The autoantibodies block TH1-mediated inflammatory autodestructive reactions and are believed to be a method by which the immune system compensates for disease (ref5). T Cell and TCR Diversity Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP). A small number of T cells lack both CD4 and CD8 (double negative, DN). Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders. Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

Panel Builder

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Single-chain VαVβ T-cell receptors function without mispairing with endogenous TCR chains.

Gene therapy

Aggen DH,Chervin AS,Schmitt TM,Engels B,Stone JD,Richman SA,Piepenbrink KH,Baker BM,Greenberg PD,Schreiber H,Kranz DM

TCR2642 was used in flow cytometry to study the ability of engineered single-chain V?V? T-cell receptors to function without mispairing with endogenous TCR chains

Sun Apr 01 00:00:00 EDT 2012

Phosphoantigen-activated V gamma 2V delta 2 T cells antagonize IL-2-induced CD4+CD25+Foxp3+ T regulatory cells in mycobacterial infection.

Blood

Gong G,Shao L,Wang Y,Chen CY,Huang D,Yao S,Zhan X,Sicard H,Wang R,Chen ZW

TCR2642 was used in flow cytometry to investigate the effect of phosphoantigen-activated V gamma 2V delta 2 T cells for interleukin-2-induced CD4+CD25+Foxp3+ T regulatory cells in mycobacterial infection

Thu Jan 22 00:00:00 EST 2009

Effects of HIV-1 peptides on T-cell receptor variable beta chain families.

Human immunology

Eick A,Larned J,Jason J

TCR2642 was used in flow cytometry to study the role of HIV-1 peptides in the expansion and deletion of TCR Vbeta families

Sun Oct 01 00:00:00 EDT 2000

The T cell receptor repertoire of CD8+CD28- T lymphocytes is dominated by expanded clones that persist over time.

Clinical and experimental immunology

Mugnaini EN,Egeland T,Spurkland A,Brinchmann JE

TCR2642 was used in flow cytometry to study the T cell receptor repertoire in CD8+CD28- T lymphocytes

Sun Aug 01 00:00:00 EDT 1999

Rapid screening of T-cell receptor (TCR) variable gene usage by multiplex PCR: application for assessment of clonal composition.

Tissue antigens

Akatsuka Y,Martin EG,Madonik A,Barsoukov AA,Hansen JA

TCR2642 was used in flow cytometry to evaluate the multiplex PCR for the screening of T-cell receptor variable gene

Mon Feb 01 00:00:00 EST 1999

Bioinformatics

Gene Aliases: TCRbeta; Tib

Entrez Gene ID: (Mouse) 21577

TCR V beta 5a Monoclonal Antibody (1C1), FITC

Product Details

TCR2642

Immunohistochemistry (Frozen) (IHC (F))

Flow Cytometry (Flow)

T-Cell Activation (TCA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!