Immunofluorescence analysis of TCR V beta 8a Antibody (I6G8) was performed using 70% confluent log phase MOLT-4 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with TCR V beta 8a (I6G8) Mouse Monoclonal Antibody (TCR1648) at 2µg/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membranous localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human, Non-human primate|
|Published species reactivity||Non-human primate, Human|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Human TCR V beta 8|
|Storage buffer||PBS with 0.5% BSA, glycerol|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|T-Cell Activation (TCA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
TCR1648 targets TCR V beta 8a in FACS, IHC(F), IP, TCA, and WB applications and shows reactivity with Human, and Non-human primate samples.
The TCR1648 immunogen is human TCR V beta 8.
The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response. TCR recognition of self-peptides has been linked to autoimmune disease. Mutant self-peptides have been associated with tumors. Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome. Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). The autoantibodies block TH1-mediated inflammatory autodestructive reactions and are believed to be a method by which the immune system compensates for disease (ref5). T Cell and TCR Diversity Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP). A small number of T cells lack both CD4 and CD8 (double negative, DN). Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders. Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Non-human primate||Not Cited||
Phosphoantigen-activated V gamma 2V delta 2 T cells antagonize IL-2-induced CD4+CD25+Foxp3+ T regulatory cells in mycobacterial infection.
TCR1648 was used in flow cytometry to investigate the effect of phosphoantigen-activated V gamma 2V delta 2 T cells for interleukin-2-induced CD4+CD25+Foxp3+ T regulatory cells in mycobacterial infection
|Gong G,Shao L,Wang Y,Chen CY,Huang D,Yao S,Zhan X,Sicard H,Wang R,Chen ZW||Blood (113:837)||2009|
T-cell receptor repertoire expression in workers with occupational asthma due to platinum salt.
TCR1648 was used in flow cytometry to study the expression of TCR repertoire in patients suffered from asthma due to platinum salt
|Raulf-Heimsoth M,Merget R,Rihs HP,Föhring M,Liebers V,Gellert B,Schultze-Werninghaus G,Baur X||The European respiratory journal (16:871)||2000|
The T cell receptor repertoire of CD8+CD28- T lymphocytes is dominated by expanded clones that persist over time.
TCR1648 was used in flow cytometry to study the T cell receptor repertoire of CD8+CD28- T lymphocytes
|Mugnaini EN,Egeland T,Spurkland A,Brinchmann JE||Clinical and experimental immunology (117:298)||1999|
Identification of an enhancer agonist cytotoxic T lymphocyte peptide from human carcinoembryonic antigen.
TCR1648 was used in flow cytometry to identify a specific peptide from human carcinoembryonic antigen to enhance the immunogenicity of tumor cell self-antigens
|Zaremba S,Barzaga E,Zhu M,Soares N,Tsang KY,Schlom J||Cancer research (57:4570)||1997|
Evidence for restricted Vbeta usage in the leukemic phase of cutaneous T cell lymphoma.
TCR1648 was used in immunohistochemistry to investigate the status of Vbeta usage in cutaneous T cell lymphoma
|Vonderheid EC,Boselli CM,Conroy M,Casaus L,Espinoza LC,Venkataramani P,Bigler RD,Hou JS||The Journal of investigative dermatology (124:651)||2005|
Immunological study on CD3 defective cutaneous T cell lymphoma cells from a patient with Sézary syndrome.
TCR1648 was used in immunohistochemistry to study the effect of CD3 surface expression on cutaneous T cell lymphoma cells
|Sano S,Matsui Y,Itami S,Yoshikawa K||Clinical and experimental immunology (113:190)||1998|
Large-scale early in vitro response to actinobacillus actinomycetemcomitans suggests superantigenic activation of T-cells.
TCR1648 was used in immunocytochemistry to study the mechanism for T cell response to Actinobacillus actinomycetemcomitans
|Zadeh HH,Nalbant A,Park K||Journal of dental research (80:356)||2001|