|Tested species reactivity||Human, Non-human primate|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Human TCR V beta 8|
|Storage buffer||PBS with 0.5% BSA, glycerol|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|T-Cell Activation (TCA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Flow Cytometry (Flow)||See 1 publications below|
TCR2648 targets TCR V beta 8a in FACS, IHC(F), IP, TCA, and WB applications and shows reactivity with Human, and Non-human primate samples.
The TCR2648 immunogen is human TCR V beta 8.
The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response. TCR recognition of self-peptides has been linked to autoimmune disease. Mutant self-peptides have been associated with tumors. Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome. Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). The autoantibodies block TH1-mediated inflammatory autodestructive reactions and are believed to be a method by which the immune system compensates for disease (ref5). T Cell and TCR Diversity Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP). A small number of T cells lack both CD4 and CD8 (double negative, DN). Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders. Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Depletion of Vbeta5.2/5.3 T cells with a humanized antibody in patients with multiple sclerosis.
TCR2648 was used in flow cytometry to study the selective depletion of Vbeta5.2/5.3 T cells in multiple sclerosis patients
|Olsson T,Edenius C,Ferm M,Samuelson P,Torrång A,Wallström E,Khademi M,Andersson M,Arfors L||European journal of neurology (9:153)||2002|