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Flow cytometry analysis of TCR V beta 8b in PBMC cells (green) compared to an isotype control (blue). Human blood was collected, combined with a hydrophilic polysaccharide, centrifuged, transferred to a conical tube and washed with PBS. 50 ul of cell solution was added to each tube at a dilution of 2x10^7 cells/ml, followed by the addition of 50 ul of isotype control and primary antibody (Product # TCR2750) at a dilution of 1:10. Cells were incubated for 30 min at 4°C and washed with a cell buffer, followed by incubation with a DyLight 488-conjugated secondary antibody for 30 min at 4°C in the dark. FACS analysis was performed using 400 ul of cell buffer.
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Human TCR Vbeta8|
|Storage buffer||PBS with 0.5% BSA, glycerol|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|T-Cell Activation (TCA)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
TCR2750 targets TCR V beta 8b in FACS, IHC(F), IP, and TCA applications and shows reactivity with Human samples.
The TCR2750 immunogen is human TCR Vbeta8.
The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response. TCR recognition of self-peptides has been linked to autoimmune disease. Mutant self-peptides have been associated with tumors. Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome. Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). The autoantibodies block TH1-mediated inflammatory autodestructive reactions and are believed to be a method by which the immune system compensates for disease (ref5). T Cell and TCR Diversity Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP). A small number of T cells lack both CD4 and CD8 (double negative, DN). Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders. Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Oligoclonal expansions of CD4+ and CD8+ T-cells in the target organ of patients with biliary atresia.
TCR2750 was used in immunocytochemistry to investigate the CD4+ and CD8+ T-cells in biliary atresia
|Mack CL,Falta MT,Sullivan AK,Karrer F,Sokol RJ,Freed BM,Fontenot AP||Gastroenterology (133:278)||2007|
Effects of HIV-1 peptides on T-cell receptor variable beta chain families.
TCR2750 was used in flow cytometry to study the role of HIV-1 peptides in TCR Vbeta families
|Eick A,Larned J,Jason J||Human immunology (61:993)||2000|
V beta-specific stimulation of human T cells by staphylococcal toxins.
TCR2750 was used in flow cytometry to investigate the dependence of staphylococcal toxin-mediated human T cell stimulation on V beta
|Kappler J,Kotzin B,Herron L,Gelfand EW,Bigler RD,Boylston A,Carrel S,Posnett DN,Choi Y,Marrack P||Science (New York, N.Y.) (244:811)||1989|
Selective accumulation of related CD4+ T cell clones in the synovial fluid of patients with rheumatoid arthritis.
TCR2750 was used in immunohistochemistry to investigate the status of CD4+ T cell clones in rheumatoid arthritis
|Striebich CC,Falta MT,Wang Y,Bill J,Kotzin BL||Journal of immunology (Baltimore, Md. : 1950) (161:4428)||1998|