Flow cytometry analysis of TCR V delta 1 on TCR V delta 1 positive PEER cells (left and middle panel) or negative control Jurkat cells (right panel). Equal numbers of cells were stained with a TCR V delta 1 (TS8.2) monoclonal antibody (Product # TCR1730) followed by FITC labeled secondary antibody, or FITC labeled secondary antibody alone. 5ul of primary antibody were used per test. All antibody incubations were performed for 30 minutes at room temperature. A representative 10,000 cells were acquired for each sample.
|Tested species reactivity||Human|
|Published species reactivity||Non-human primate, Human, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human TCR V delta 1|
|Storage buffer||PBS with glycerol, 0.5% BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1-2 µg/test|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|T-Cell Activation (TCA)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
TCR1730 targets TCR V delta 1 in FACS, IHC (F), and TCA applications and shows reactivity with Human samples.
The TCR1730 immunogen is human TCR V delta 1.
The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response. TCR recognition of self-peptides has been linked to autoimmune disease. Mutant self-peptides have been associated with tumors. Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome. Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). The autoantibodies block TH1-mediated inflammatory autodestructive reactions and are believed to be a method by which the immune system compensates for disease (ref5). T Cell and TCR Diversity Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP). A small number of T cells lack both CD4 and CD8 (double negative, DN). Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders. Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Functional dichotomy of V¿2 ¿¿ T cells in chronic hepatitis C virus infections: role in cytotoxicity but not for IFN-¿ production.
TCR1730 was used in flow cytometry to study chronic hepatitis C virus infections and the functional dichotomy of V-delta2 gamma-delta T cells and their role in cytotoxicity and not IFN-gamma production
|Yin W,Tong S,Zhang Q,Shao J,Liu Q,Peng H,Hu H,Peng M,Hu P,Ren H,Tian Z,Zhang D||Scientific reports (6:null)||2016|
|Not Applicable||Not Cited||
Molecular Analysis of Lipid-Reactive V¿1 ¿¿ T Cells Identified by CD1c Tetramers.
TCR1730 was used in flow cytometry to study CD1c tetramers that identify molecular analysis of lipid-reactive V-delta-1 gamma-delta T Cells
|Roy S,Ly D,Castro CD,Li NS,Hawk AJ,Altman JD,Meredith SC,Piccirilli JA,Moody DB,Adams EJ||Journal of immunology (Baltimore, Md. : 1950) (196:1933)||2016|
Enhanced functions of peripheral ¿¿ T cells in chronic hepatitis B infection during interferon ¿ treatment in vivo and in vitro.
TCR1730 was used in flow cytometry to examine the phenotype and function of peripheral gammadelta T cells in patients with chronic hepatitis B during pegylated-interferon-alpha treatment.
|Chen M,Hu P,Ling N,Peng H,Lei Y,Hu H,Zhang D,Ren H||PloS one (10:null)||2015|
¿¿ T cells are involved in acute HIV infection and associated with AIDS progression.
TCR1730 was used in flow cytometry to identify the role of GammaDelta T cells in acute HIV infection
|Li Z,Li W,Li N,Jiao Y,Chen D,Cui L,Hu Y,Wu H,He W||PloS one (9:null)||2014|
|Non-human primate||Not Cited||
Characterization of ¿¿T cells in naïve and HIV-infected chimpanzees and their responses to T-cell activators in vitro.
TCR1730 was used in flow cytometry to study the in vitro response of gammadelta T-cell subsets from HIV1-infected and control chimpanzees to T-cell activators
|Hodara VL,Parodi LM,Chavez D,Smith LM,Lanford R,Giavedoni LD||Journal of medical primatology (43:258)||2014|
Novel bispecific antibodies increase ¿¿ T-cell cytotoxicity against pancreatic cancer cells.
TCR1730 was used in flow cytometry to study the cytotoxicity of gammadelta T-cells towards pancreatic cancer cells mediated by bispecific antibodies that selectively recruit to tumor antigens
|Oberg HH,Peipp M,Kellner C,Sebens S,Krause S,Petrick D,Adam-Klages S,Röcken C,Becker T,Vogel I,Weisner D,Freitag-Wolf S,Gramatzki M,Kabelitz D,Wesch D||Cancer research (74:1349)||2014|
|Non-human primate||Not Cited||
Phosphoantigen/IL2 expansion and differentiation of V¿2V¿2 T cells increase resistance to tuberculosis in nonhuman primates.
TCR1730 was used in flow cytometry to study the increased Tuberculosis resistance of non-human primates following phoshoantigen/IL-2 mediated expansion and differentiation of VgammaVdelta2 T-cells
|Chen CY,Yao S,Huang D,Wei H,Sicard H,Zeng G,Jomaa H,Larsen MH,Jacobs WR,Wang R,Letvin N,Shen Y,Qiu L,Shen L,Chen ZW||PLoS pathogens (9:null)||2013|
Characterization and immunotherapeutic potential of gammadelta T-cells in patients with glioblastoma.
TCR1730 was used in flow cytometry to investigate the role of gammadelta T-cells in patients with glioblastoma
|Bryant NL,Suarez-Cuervo C,Gillespie GY,Markert JM,Nabors LB,Meleth S,Lopez RD,Lamb LS||Neuro-oncology (11:357)||2009|
A rapid crosstalk of human gammadelta T cells and monocytes drives the acute inflammation in bacterial infections.
TCR1730 was used in flow cytometry to investigate the importance of the crosstalk between V gamma 9/V delta 2 T cells and monocytes in immunological response to microbial infection
|Eberl M,Roberts GW,Meuter S,Williams JD,Topley N,Moser B||PLoS pathogens (5:null)||2009|
Selective activation of TCR-gammadelta+ cells in endemic Burkitt's lymphoma.
TCR1730 was used in flow cytometry to investigate different T-cell compositions and activation status in Plasmodium falciparum-exposed Ghanaian children with and without endemic Burkitt lymphoma
|Futagbi G,Welbeck JE,Tetteh JK,Hviid L,Akanmori BD||Malaria journal (6:null)||2007|
Recognition of nonpeptide antigens by human V gamma 9V delta 2 T cells requires contact with cells of human origin.
TCR1730 was used in flow cytometry to study the mechanism for nonpeptide antigen recognition by human Vgamma9Vdelta2 T cells
|Green AE,Lissina A,Hutchinson SL,Hewitt RE,Temple B,James D,Boulter JM,Price DA,Sewell AK||Clinical and experimental immunology (136:472)||2004|
|Non-human primate||Not Cited||
Inhibition of adaptive Vgamma2Vdelta2+ T-cell responses during active mycobacterial coinfection of simian immunodeficiency virus SIVmac-infected monkeys.
TCR1730 was used in flow cytometry to study the effect of mycobacterial coinfection on Vgamma2Vdelta2+ T-cell responses
|Zhou D,Lai X,Shen Y,Sehgal P,Shen L,Simon M,Qiu L,Huang D,Du GZ,Wang Q,Letvin NL,Chen ZW||Journal of virology (77:2998)||2003|
CD1d-lipid antigen recognition by the ¿¿ TCR.
TCR1730 was used in immunocytochemistry to study the mechanism by which the gamma delta TCR recognizes CD1d loaded with alpha-galactosylceramide
|Uldrich AP,Le Nours J,Pellicci DG,Gherardin NA,McPherson KG,Lim RT,Patel O,Beddoe T,Gras S,Rossjohn J,Godfrey DI||Nature immunology (14:1137)||2013|
The bisphosphonate acute phase response: rapid and copious production of proinflammatory cytokines by peripheral blood gd T cells in response to aminobisphosphonates is inhibited by statins.
TCR1730 was used in immunocytochemistry to characterize the acute phase response by bisphosphonates
|Hewitt RE,Lissina A,Green AE,Slay ES,Price DA,Sewell AK||Clinical and experimental immunology (139:101)||2005|
Extensive expansion of primary human gamma delta T cells generates cytotoxic effector memory cells that can be labeled with Feraheme for cellular MRI.
TCR1730 was used in blocking or activating experiment to develop a method for the extensive expansion of gamma delta T-cells to be labelled with Feraheme for use in cellular MRI studies
|Siegers GM,Ribot EJ,Keating A,Foster PJ||Cancer immunology, immunotherapy : CII (62:571)||2013|
Maternal Foxp3 expressing CD4+ CD25+ and CD4+ CD25- regulatory T-cell populations are enriched in human early normal pregnancy decidua: a phenotypic study of paired decidual and peripheral blood samples.
TCR1730 was used in immunohistochemistry to identify relationship between T-cell populations and pregnancy
|Dimova T,Nagaeva O,Stenqvist AC,Hedlund M,Kjellberg L,Strand M,Dehlin E,Mincheva-Nilsson L||American journal of reproductive immunology (New York, N.Y. : 1989) (66 Suppl 1:44)||2011|
Cutaneous extranodal NK/T-cell lymphoma: a clinicopathologic study of 5 patients with array-based comparative genomic hybridization.
TCR1730 was used in immunohistochemistry to study the clinicopathology of ENK/T lymphoma with cutaneous involvement
|Berti E,Recalcati S,Girgenti V,Fanoni D,Venegoni L,Vezzoli P||Blood (116:165)||2010|
Flexible migration program regulates gamma delta T-cell involvement in humoral immunity.
TCR1730 was used in immunohistochemistry to investigate the migration of gamma delta T cells
|Brandes M,Willimann K,Lang AB,Nam KH,Jin C,Brenner MB,Morita CT,Moser B||Blood (102:3693)||2003|
Monoclonal antibodies to T cell receptor gamma/delta complex react with human endometrial glandular epithelium.
TCR1730 was used in immunohistochemistry to test the reactivity of monoclonal antibodies to T cell receptor gamma/delta complex
|Yeh CJ,Bulmer JN,Hsi BL,Tian WT,Rittershaus C,Ip SH||Placenta (11:253)||1990|
Homing and function of human skin gammadelta T cells and NK cells: relevance for tumor surveillance.
TCR1730 was used in ELISA to investigate the role of human skin gammadelta T and natural killer cells
|Ebert LM,Meuter S,Moser B||Journal of immunology (Baltimore, Md. : 1950) (176:4331)||2006|